Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3375 |
| PDBID: | 7B2M |
| Chains: | D_B |
| Organism: | Homo sapiens, Lama glama |
| Method: | EM |
| Resolution (Å): | 3.39 |
| Reference: | 10.4049/jimmunol.2100647 |
| Antibody | |
| Antibody: | Anti-C4b E3 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | complement C4b alpha chain |
| Antigen mutation: | Yes |
| Durg Target: | P0C0L4; P0C0L5; |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >7B2M_D|Chain D|Anti-C4b nanobody E3|Lama glama (9844) EVQLVESGGGLVQAGGSLRLSCAASGSDFSANAVGWYRQAPGKQRVVVASISSTGNTKYSNSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCWLFRFGIENYWGQGTQVTVSSHGSGLVPRGSGGGHHHHHH |
Antigen
Chain: B
Mutation: T522S
| >7B2M_B|Chain B|Complement C4 alpha chain|Homo sapiens (9606) NVNFQKAINEKLGQYASPTAKRCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFSVVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKANSFLGEKASAGLLGAHAAAITAYALSLTKAPVDLLGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQASAWLTRQGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQIRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: ASN32 ALA33 TYR37 GLN44 ARG45 VAL47 SER50 SER52 SER53 THR54 ASN56 LYS58 TYR59 SER60 ASN61 TRP96 PHE98 PHE100 GLY101 ILE102 ASN104 TRP106 B: GLU763 GLU764 ASP765 LEU766 ILE767 ASP768 ASP770 ASP771 ILE772 PRO773 ARG831 GLU832 PHE833 LEU859 ARG916 PHE919 GLU920 PHE921 PRO922 VAL923 GLY924 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
