Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3369 |
| PDBID: | 7AMS |
| Chains: | HL_B |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.42 |
| Reference: | 10.21203/rs.3.rs-1475171/v1 |
| Antibody | |
| Antibody: | KFN mutant of HuJovi-1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | T cell receptor β-chain constant domains 2 (TRBC2) |
| Antigen mutation: | No |
| Durg Target: | P01850 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7AMS_H|Chain C[auth H]|Human Jovi-1 Fab, KFN mutant, heavy chain|Homo sapiens (9606) QVQLVQSGAEVKKPGASVKVSCKASGYKFTGFVMHWVRQAPGQGLEWMGFINPYNDDIQSNERFRGRVTMTRDTSISTAYMELSRLRSDDTAVYYCARGNGYNFDGAYRFFDFWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: L
Mutation: NULL
| >7AMS_L|Chain D[auth L]|Human Jovi-1 Fab, KFN mutant, light chain|Homo sapiens (9606) DIVMTQSPLSLPVTPGEPASISCRSSQRLVHSNGNTYLHWYLQKPGQSPRLLIYRVSNRFPGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCSQSTHVPYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >7AMS_B|Chain B|Human A6 T-cell receptor beta chain TRBC2|Homo sapiens (9606) MNAGVTQTPKFQVLKTGQSMTLQCAQDMNHEYMSWYRQDPGMGLRLIHYSVGAGITDQGEVPNGYNVSRSTTEDFPLRLLSAAPSQTSVYFCASRPGLAGGRPEQYFGPGTRLTVTEDLKNVFPPEVAVFEPSEAEISHTQKATLVCLATGFYPDHVELSWWVNGKEVHSGVCTDPQPLKEQPALNDSRYALSSRLRVSATFWQDPRNHFRCQVQFYGLSENDEWTQDRAKPVTQIVSAEAWGRAD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLY26 TYR27 LYS28 PHE32 TYR54 ARG98 ASN100 GLY101 TYR102 ASN103 ASP105 GLY106 ALA107 PHE110 L: ASN33 ASN35 TYR37 TYR54 ARG55 ARG59 PHE60 PRO61 GLY62 B: GLU116 ASP117 LYS119 ASN120 PHE122 GLN181 ASP186 ARG188 GLU223 TRP224 THR225 GLN226 ASP227 ARG228 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
