Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3360 |
| PDBID: | 7AHV |
| Chains: | AB_H |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.11 |
| Reference: | 10.1182/blood.2020010331 |
| Antibody | |
| Antibody: | Mim8 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Coagulation factor IX |
| Antigen mutation: | No |
| Durg Target: | P00740 |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >7AHV_A|Chain A|anti-FIXa Fab of mim8 heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGRSLRLSCAASGFTFHDYAMHWVRQVPGKGLEWVSGISWRGDIGGYVKSVKGRFTISRDNAKNSLYLQMNSLRAEDTALYYCVKSYGSGSFYNAFDSWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKY |
Light Chain: B
Mutation: NULL
| >7AHV_B|Chain B|anti-FIXa Fab of mim8 light chain|Homo sapiens (9606) DIQMTQSPSTLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKFLIYKASKLERGTPSRFSGSGDGTEFSLTISSLQPDDFATYYCLEYSSYIRTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDAALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: H
Mutation: NULL
| >7AHV_H|Chain D[auth H]|Coagulation factor IX|Homo sapiens (9606) VVGGEDAKPGQFPWQVVLNGKVDAFCGGSIVNEKWIVTAAHCVETGVKITVVAGEHNIEETEHTEQKRNVIRIIPHHNYNAAINKYNHDIALLELDEPLVLNSYVTPICIADKEYTNIFLKFGSGYVSGWGRVFHKGRSALVLQYLRVPLVDRATCLRSTKFTIYNNMFCAGFHEGGRDSCQGDSGGPHVTEVEGTSFLTGIISWGEECAMKGKYGIYTKVSRYVNWIKEKTKLT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition A: HIS30 ASP31 SER52 TRP53 ARG54 ASP56 ILE57 SER102 GLY103 SER104 PHE105 TYR106 ASN107 B: TRP32 LYS50 LYS53 TYR91 SER92 H: ILE96 ARG165 ALA166 THR167 LEU169 ARG170 SER171 THR172 LYS173 PHE174 THR175 TYR177 HIS185 LYS224 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
