Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3340 |
| PDBID: | 7A17 |
| Chains: | F_E |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.73 |
| Reference: | 10.7554/eLife.64922 |
| Antibody | |
| Antibody: | 13015 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Isoform 2 of Synaptojanin-1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: F
Mutation: NULL
| >7A17_F|Chain F|Nanobody 13015|Lama glama (9844) QVQLVESGGGFAQAGGSLRLSCAASGSTFRFRAMGWFRQAPGKEREFVAGISWSGSTKYTDSVKGRFTISRDNAKNTVHLQMNNLTPEDTAVYYCAQSRAIEADDSRGYDYWGQGTQVTVSSHHH |
Antigen
Chain: E
Mutation: NULL
| >7A17_E|Chain C, E|Isoform 2 of Synaptojanin-1|Homo sapiens (9606) KPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVSASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFIEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQVFRGFLEGKVTFAPTYKYDLFSDDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: GLN1 SER27 THR28 ARG30 PHE31 ARG32 TRP53 SER54 ARG99 ALA100 ILE101 GLU102 ASP110 TYR111 E: VAL626 LEU627 LEU628 ALA629 GLU631 ILE642 PRO644 ALA647 PRO648 ILE650 ARG651 ASP652 VAL653 ALA654 VAL655 LEU677 PRO714 MET715 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
