Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3335 |
| PDBID: | 7A0W |
| Chains: | EF_B |
| Organism: | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, Gallus gallus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.04 |
| Reference: | Not available or To be published |
| Antibody | |
| Antibody: | chimeric F6 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Na(+)/H(+) antiporter NhaA |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >7A0W_E|Chain C, E|chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain|Gallus gallus (9031) AVTLDESGGGLQTPGGGLSLVCKASGFTFSDYGMGWVRQAPDKGLEWVAGIYTTGSGTRYGAAVKGRATISRDNGQSTVRLQLNNLRAEDTGTYYCAKSTGRDYYGGGIDAWGHGTEVIVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTSGQAGQHHHHHHGAYPYDVPDYAS |
Light Chain: F
Mutation: NULL
| >7A0W_F|Chain D, F|chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain|Gallus gallus (9031) ALTQPSSVSANPGGTVKITCSGGTYSYGWFQQKSPGSAPVTVIYWNDKRPSNIPSRFSGSKSGSTHTLTITGVRAEDEAVYFCGSADSSGTAIFGAGTTLTVLRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSLPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >7A0W_B|Chain A, B|Na(+)/H(+) antiporter NhaA|Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (99287) MKHLHRFFSSDASGGIILIIAAALAMLMANMGATSGWYHDFLETPVQLRVGALEINKNMLLWINDALMAVFFLLIGLEVKRELMQGSLASLRQAAFPVIAAIGGMIVPALLYLAFNYSDPVTREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALAIIDDLGAIVIIALFYTSDLSIVSLGVAAFAIAVLALLNLCGVRRTGVYILVGAVLWTAVLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSLQGVTIDGLTSMLPLGIIAGLLIGKPLGISLFCWLALRFKLAHLPQGTTYQQIMAVGILCGIGFTMSIFIASLAFGNVDPELINWAKLGILIGSLLSAVVGYSWLRARLNAPASENLYFQGGRGSHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: ASP31 TYR32 TYR52 THR54 SER56 GLY57 THR58 ARG59 THR100 GLY101 ARG102 ASP103 TYR104 TYR105 F: THR24 TYR25 SER26 TRP45 ASN46 ASP47 LYS48 LYS61 SER62 GLY63 SER64 SER88 SER89 GLY90 B: HIS39 GLU43 SER118 ASP119 PRO120 VAL121 THR122 ARG123 GLU124 GLN277 GLY278 SER286 MET287 LEU288 LEU348 ALA349 GLY351 ASN352 VAL353 ASP354 PRO355 GLU356 LEU357 TRP360 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
