Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3328 |
| PDBID: | 6ZRV |
| Chains: | B_A |
| Organism: | Homo sapiens, Vicugna pacos |
| Method: | XRD |
| Resolution (Å): | 1.88 |
| Reference: | 10.3390/ijms21165859 |
| Antibody | |
| Antibody: | Nb93 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Plasminogen activator inhibitor 1 |
| Antigen mutation: | Yes |
| Durg Target: | P05121 |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >6ZRV_B|Chain B|VHH-s-a93 (Nb93)|Vicugna pacos (30538) QVQLVESGGGLVQPGGSLRLSCAASGFSLDNYAIGWFRQAPGKEREGVSCISSSDGSTYYTDSVEGRFTISRDNAKNTVYLQMNSLKPDDTAVYYCAADYGSSWCTFNGMDYWGQGTQVTVSS |
Antigen
Chain: A
Mutation: W175F
| >6ZRV_A|Chain A|Plasminogen activator inhibitor 1|Homo sapiens (9606) VHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPYGVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAIFVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAVDQLTRLVLVNALYFNGQFKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPDGHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILSAQLISHWKGNMTRLPRLLVLPKFSLETEVDLRKPLENLGMTDMFRQFQADFTSLSDQEPLHVAQALQKVKIEVNESGTVASSSTAVIVSARMAPEEIIMDRPFLFVVRHNPTGTVLFMGQVMEP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: PHE37 GLN39 GLY42 LYS43 GLU44 ARG45 GLU46 GLY47 VAL48 SER49 CYS50 TYR59 TYR60 THR61 ASP62 GLU65 ASP99 TYR100 SER102 SER103 TRP104 CYS105 THR106 PHE107 ASN108 GLY109 ASP111 TRP113 A: THR177 PRO180 ASP181 SER182 THR205 ASN206 LYS207 ARG268 PRO270 ARG271 SER336 SER337 THR339 ALA340 VAL341 ILE342 VAL343 SER344 ARG346 MET347 ALA348 PRO349 GLU350 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
