Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3327 |
| PDBID: | 6ZPL |
| Chains: | F_A |
| Organism: | Homo sapiens, Acetivibrio thermocellus ATCC 27405, unidentified |
| Method: | XRD |
| Resolution (Å): | 3.94 |
| Reference: | 10.1038/s41586-021-03274-z |
| Antibody | |
| Antibody: | Sb_GlyT1#7 synthetic-Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Sodium- and chloride-dependent glycine transporter 1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: F
Mutation: NULL
| >6ZPL_F|Chain D[auth E], E[auth F]|Sybody Sb_GlyT1#7|unidentified (32644) QVQLVESGGGLVQAGGSLRLSCAASGFPVYAYEMYWYRQAPGKEREWVAAISSSGTWAGYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCNVKDWGASWAYYDYWGQGTQVTVS |
Antigen
Chain: A
Mutation: NULL
| >6ZPL_A|Chain A, B|Sodium- and chloride-dependent glycine transporter 1|Homo sapiens (9606) TKRDQNLKRGNWGNQIEFVLTSVGYAVGLGNVWRFPYLCYRNGGGAFMFPYFIMLIFCGIPAFFMELSFGQFASQGCLGVWRISPMFKGVGYGMMVVSTYIGIYYNVVICIAFYYFFSSMTHVLPWAYCNNPWNTHDCAGVLDASNLTHSLQRTSPSEEYWRLYVLKLSDDIGNFGEVRLPLLGCLGVAWLVVFLCLIRGVKSSGKVVYFTATFPYVVLTILFVRGVTLEGAFDGIMYYLTPQWDKILEAKVWGDAASQAFYSLGCAWGGLITMASYNKFHNNCYRDSVIISITNCATSVYAGFVIFSILGFMANHLGVDVSRVADHGPGLAFVAYPEALTLLPISPLWSLLFFFMLILLGLGTQFCLLETLVTAIVDEVGNEWILQKKTYVTLGVAVAGFLLGIPLTSQAGIYWLLLMDNYAASFSLVVISCIMCVAIMYIYGHRNYFQDIQMMLGFPPPLFFQICWRFVSPAIIFFILVFTVIQYQPITYNHYQYPGWAVAIGFLMALSSVLCIPLYAMFRLARTDGDTLLQRLKNATKPSRDWGPALLEHRTGRYAPTIAPSPEDGFEVQPLH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: GLY26 PHE27 PRO28 TYR30 ALA31 TYR32 GLU33 SER52 SER53 SER54 THR56 TRP57 ASN74 ASP100 TRP101 GLY102 ALA103 SER104 TRP105 A: THR212 HIS213 VAL214 LEU215 ALA218 TYR219 GLY231 VAL232 LEU233 ASP234 ARG261 ARG333 VAL335 THR336 LEU337 GLU338 GLY339 ALA340 PHE420 ASN423 HIS424 THR449 LEU450 LEU451 PRO452 ILE453 SER454 PRO455 LEU456 LEU459 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
