Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3312 |
| PDBID: | 6ZE1 |
| Chains: | B_A |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.71 |
| Reference: | 10.1038/s41589-021-00844-0 |
| Antibody | |
| Antibody: | G11a Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | cystic fibrosis transmembrane conductance regulator (CFTR) |
| Antigen mutation: | No |
| Durg Target: | P13569 |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >6ZE1_B|Chain B|G11a nanobody|Lama glama (9844) QVQLQESGGGVVGPGGSLRLACAFSGRTFSDYWMAWFRQTPGEERDFVAAISRSGITTSYGDFVEGRFTITRDNAKNTVNLQMNFLKPEDTADYYCAAGTSSFLRREYDYWGQGTQVTVSSAAAHHHHHHGAAEQKLISEEDLNGAA |
Antigen
Chain: A
Mutation: NULL
| >6ZE1_A|Chain A|Cystic fibrosis transmembrane conductance regulator|Homo sapiens (9606) SLTTTEVVMENVTAFWEEGFGELFEKAKQNNNNRKTSNGDDSLFFSNFSLLGTPVLKDINFKIERGQLLAVAGSTGAGKTSLLMMIMGELEPSEGKIKHSGRISFCPQFSWIMPGTIKENIIFGVSYDEYRYRSVIKACQLEEDISKFPEKDNTVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEIFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLQPDFSSKLMG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: ASP31 PHE47 SER52 ARG53 SER54 GLY55 ILE56 THR57 THR58 SER59 TYR60 THR100 SER101 SER102 PHE103 LEU104 ARG105 ARG106 A: ASN423 GLY424 ASP425 SER427 LEU428 PHE429 PHE430 SER431 ASN432 LEU435 LEU436 MET470 GLY473 GLU474 LEU475 GLU476 PRO477 SER478 GLU479 LYS481 SER485 ARG487 ILE488 ASP567 LEU568 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
