Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3289 |
| PDBID: | 6Z06 |
| Chains: | HL_A |
| Organism: | Myodes glareolus, Orthohantavirus puumalaense |
| Method: | XRD |
| Resolution (Å): | 3.50 |
| Reference: | 10.7554/eLife.58242 |
| Antibody | |
| Antibody: | 4G2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Puumala virus Gc envelope polyprotein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6Z06_H|Chain A[auth H]|Fab 4G2 Heavy chain|Myodes glareolus (447135) TGQVQMKESGGGLVQPGKSLKLSCAASGFTFSDFWMSWVRQPSGKGLEWVARINTNGDTTHYTDDMKGRFTISRDNAKTTLYLEMSPLKSEDTAMYYCTRDRLGPFDYWGQGTTVTVSSATTKGPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSQTVTCNVAHPASSTKVDKKIVPRDCGGTKHHHHHH |
Light Chain: L
Mutation: NULL
| >6Z06_L|Chain B[auth L]|Fab 4G2 Light chain|Myodes glareolus (447135) TGDIVMTQSPAFPPVSLGDTVTITCQASQSVYKNLAWYQQKPGKSPRLLIFGMSSLADGVPSRFSASGSDKQYSLKIRGLQPEDAAIYYCQQHYIAPYTFGAGTRLEIKRTDAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >6Z06_A|Chain C[auth A]|Envelope polyprotein|Puumala orthohantavirus (3052493) GPGETQNLNSGWTDTAHGSGIIPMRTDLELDFSLPSSASYTYRRQLQNPANEQEKIPFHLQISKQVIHAEIQHLGHWMDGTFNLKTAFHCYGSCEKYAYPWQTAGCFIEKDYEYESGWGCNPPDCPGVGTGCTACGVYLDKLKSVGKAFKIVSLRYTRKACIQLGTEQTCKSVDSNDCLVTTSVKVCLIGTVSKFQPSDTLLFLGPLEQGGLIFKQWCTTTCQFGDPGDIMSTPVGMKCPELNGSFRKKCAFATTPVCQFDGNTLSGYKRMIATKDSFQSFNVTEPHISASSLEWIDPDSSLRDHINVIVGRDLSFQDLSETPCQVDLATTSIDGAWGSGVGFNLVCSVSLTECSTFLTSIKACDSAMCYGSTTANLLRGQNTVHIVGKGGHSGSKFMCCHDTKCSSTGLVAAAPHLDRVTGYNQADSDKIFDDGAPECGISCWFTKSGEGTETSQVAPA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR28 ASP31 PHE32 TRP33 THR57 HIS59 ASP99 ARG100 LEU101 PRO103 L: TYR30 LYS31 ASN32 PHE49 GLY50 SER52 SER53 LEU54 ASP56 SER60 HIS91 TYR92 ILE93 ALA94 TYR96 A: HIS723 GLU725 ILE726 GLN727 LYS805 ILE806 VAL807 SER808 LEU809 ARG810 ASP829 SER830 ASN831 ILE844 GLY845 GLN851 PRO852 SER853 ASP854 LYS870 THR874 THR875 SER944 ALA945 SER946 SER947 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
