Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3264 |
| PDBID: | 6YIO |
| Chains: | HL_B |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.83 |
| Reference: | 10.1038/s43018-020-00133-0 |
| Antibody | |
| Antibody: | RG6292 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Vopikitug(Phase-I) |
| Antigen | |
| Antigen: | Interleukin-2 receptor subunit alpha (CD25) |
| Antigen mutation: | No |
| Durg Target: | P01589 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6YIO_H|Chain B[auth H]|FAB FRAGMENT HEAVY CHAIN|Homo sapiens (9606) QVQLVQSGAEVKKPGSSVKVSCKASGGTFSSLAISWVRQAPGQGLEWMGGIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARGGSVSGTLVDFDIWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDK |
Light Chain: L
Mutation: NULL
| >6YIO_L|Chain C[auth L]|FAB FRGAMENT LIGHT CHAIN|Homo sapiens (9606) DIQMTQSPSTLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIYKASSLESGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQQYNIYPITFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >6YIO_B|Chain A[auth B]|Interleukin-2 receptor subunit alpha|Homo sapiens (9606) ELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGEMETSQFPGEEKPQASPEGRPESETSCAHHHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: THR28 SER30 SER31 LEU32 ALA33 ILE52 ILE54 PHE55 GLU74 SER75 ARG98 GLY100 SER101 VAL102 SER103 GLY104 LEU106 ASP108 ASP110 L: TRP32 TYR49 LYS50 GLU55 TYR91 ASN92 B: THR14 PHE15 LYS16 ALA17 MET18 LEU100 TYR128 TYR129 GLN130 CYS131 TYR135 ARG136 ALA137 LEU138 HIS139 ARG140 GLY141 PRO142 ALA143 GLU144 LEU161 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
