Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 326 |
| PDBID: | 2QAD |
| Chains: | HG_E |
| Organism: | Human immunodeficiency virus 1, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.30 |
| Reference: | 10.1126/science.1145373 |
| Antibody | |
| Antibody: | 412d Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 YU2 gp120 |
| Antigen mutation: | No |
| Durg Target: | P35961; P35961; P12488; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >2QAD_H|Chain D, H|anti-HIV-1 antibody 412d heavy chain|Homo sapiens (9606) EVQLVQSGAEVKKPGSSVKVSCKASGGTFSNYAINWVRQAPGQGLEWMGGIIPIFNIAHYAQRFQGRVSITADESTSTAYMELSSLRSEDTAVFYCASPYPNDYNDYAPEEGMSWYFDLWGRGTLVTVSPASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: G
Mutation: NULL
| >2QAD_G|Chain C, G|anti-HIV-1 antibody 412d light chain|Homo sapiens (9606) DIQMTQSPSTLSASVGDRVTITCRASQSISNWLAWYQQKPGRAPKLLMYKASSLKSGVPSRFSGSGSGTEFTLTISSLQSDDFATYYCQQHDSSPYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKLYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: E
Mutation: NULL
| >2QAD_E|Chain A, E|Envelope glycoprotein gp160|Human immunodeficiency virus 1 (11676) VTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTGGSVITQACPKVSFEPIPIHYCAPAGFAILKCNDKKFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIVIRSENFTNNAKTIIVQLNESVVINCTRPNNNTRKSINIGPGRALYTTGEIIGDIRQAHCNLSKTQWENTLEQIAIKLKEQFGNNKTIIFNPSSGGDPEIVTHSFNCGGEFFYCNSTQLFTWNDTRKLNNTGRNITLPCRIKQIINMWQEVGKAMYAPPIRGQIRCSSNITGLLLTRDGGKDTNGTEIFRPGGGDMRDNWRSELYKYKVVKIE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| G: TRP32 HIS91 ASP92 SER93 SER94 TYR96 H: PRO52 ILE53 PHE54 ILE56 HIS58 E: CYS119 VAL120 LEU122 VAL200 ILE201 THR202 GLN203 ALA204 CYS205 PRO206 LYS207 ILE322 GLY324 ASP325 ILE326 ARG327 GLN422 ILE423 LYS432 ALA433 MET434 ALA436 PRO437 PRO438 ILE439 ARG440 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
