Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3256 |
| PDBID: | 6Y9B |
| Chains: | HL_C |
| Organism: | Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 2.97 |
| Reference: | 10.1074/jbc.RA120.013690 |
| Antibody | |
| Antibody: | 120.545 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Metalloreductase STEAP1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6Y9B_H|Chain C[auth H], D[auth I], E[auth J]|Fab120.545 heavy chain|Mus musculus (10090) DVQVQESGPGLVKPSQSLSLTCTVTGYSITSDYAWNWIRQFPGNKLEWMGYISNSGSTSYNPSLKSRISITRDTSKNQFFLQLISVTTEDTATYYCARERNYDYDDYYYAMDYWGQGTTLTVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTHHHHHH |
Light Chain: L
Mutation: NULL
| >6Y9B_L|Chain B[auth L], F[auth M], G[auth N]|Fab120.545 light chain|Mus musculus (10090) DIVMSQSPSSLAVSVGEKVTMSCKSSQSLLYRSNQKNYLAWYQQKPGQSPKLLIYWASTRESGVPDRFTGSGSGTDFTLTISSVKAEDLAVYYCQQYYNYPRTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >6Y9B_C|Chain A, H[auth B], I[auth C]|Metalloreductase STEAP1|Homo sapiens (9606) MGSESRKDITNQEELWKMKPRRNLEEDDYLHKDTGETSMLKRPVLLHLHQTAHADEFDCPSELQHTQELFPQWHLPIKIAAIIASLTFLYTLLREVIHPLATSHQQYFYKIPILVINKVLPMVSITLLALVYLPGVIAAIVQLHNGTKYKKFPHWLDKWMLTRKQFGLLSFFFAVLHAIYSLSYPMRRSYRYKLLNWAYQQVQQNKEDAWIEHDVWRMEIYVSLGIVGLAILALLAVTSIPSVSDSLTWREFHYIQSKLGIVSLLLGTIHALIFAWNKWIDIKQFVWYTPPTFMIAVFLPIVVLIFKSILFLPCLRKKILKIRHGWEDVTKINKTEICSQLAAAGAAWSHPQFEKGAAWSHPQFEKGAAWSHPQFEKGAA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR31 SER33 ASN34 GLN35 LYS36 SER58 THR59 ASN99 TYR100 H: TYR51 SER53 SER55 SER57 THR58 SER59 LYS65 TYR102 ASP103 TYR104 ASP105 ASP106 TYR107 TYR108 C: SER101 HIS102 GLN103 GLN104 TYR105 TYR107 TYR190 LYS191 ASN194 TRP195 TYR197 GLN198 GLN201 GLN202 ASN203 LYS204 GLU205 ALA207 ILE209 LYS281 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
