Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3255 |
| PDBID: | 6Y92 |
| Chains: | CD_B |
| Organism: | Homo sapiens |
| Method: | EM |
| Resolution (Å): | 4.73 |
| Reference: | 10.1126/science.abb8008 |
| Antibody | |
| Antibody: | Ofatumumab Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Ofatumumab(Approved) |
| Antigen | |
| Antigen: | B-lymphocyte antigen CD20 |
| Antigen mutation: | No |
| Durg Target: | P11836 |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >6Y92_C|Chain C, D[auth H]|Ofatumumab Fab heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGRSLRLSCAASGFTFNDYAMHWVRQAPGKGLEWVSTISWNSGSIGYADSVKGRFTISRDNAKKSLYLQMNSLRAEDTALYYCAKDIQYGNYYYGMDVWGQGTTVTVSSASTKGPSVFPLAPGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP |
Light Chain: D
Mutation: NULL
| >6Y92_D|Chain B[auth D], E[auth L]|Ofatumumab Fab light chain|Homo sapiens (9606) EIVLTQSPATLSLSPGERATLSCRASQSVSSYLAWYQQKPGQAPRLLIYDASNRATGIPARFSGSGSGTDFTLTISSLEPEDFAVYYCQQRSNWPITFGQGTRLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR |
Antigen
Chain: B
Mutation: NULL
| >6Y92_B|Chain A, F[auth B]|B-lymphocyte antigen CD20|Homo sapiens (9606) TQSFFMRESKTLGAVQIMNGLFHIALGGLLMIPAGIYAPICVTVWYPLWGGIMYIISGSLLAATEKNSRKCLVKGKMIMNSLSLFAAISGMILSIMDILNIKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQELVIAGIVENEWKR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: SER30 TYR32 ARG91 SER92 ASN93 TRP94 C: THR28 ASN30 ASP31 SER52 TRP53 ASN54 SER57 ASN74 GLN101 TYR102 GLY103 ASN104 TYR105 TYR107 B: ILE76 TYR77 SER144 HIS145 PRO160 TYR161 ILE162 ASN163 TYR165 ASN166 GLU168 PRO169 ALA170 ASN171 PRO172 SER173 GLU174 LYS175 TYR184 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
