Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3251 |
| PDBID: | 6XZW |
| Chains: | HL_D |
| Organism: | Neisseria meningitidis MC58, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.40 |
| Reference: | 10.1371/journal.ppat.1008882 |
| Antibody | |
| Antibody: | 4B3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | meningococcal vaccine antigen fHbp |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6XZW_H|Chain C[auth H]|Fab 4B3 (heavy chain)|Homo sapiens (9606) QVQLVQSGGGLVKPGGSLRLSCAASGFPFSSYYMSWIRQAPGKGLEWVSDINNSGNVKEYADFVKGRLTISRDNVKNSMYLHMNSLRVEDTAVYYCARNRGRFDVWGQGTLVTVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDK |
Light Chain: L
Mutation: NULL
| >6XZW_L|Chain B[auth L]|Fab 4B3 (light chain)|Homo sapiens (9606) EIVMTQSPGTLSLSPGETATLSCRASQMISSPFLAWYQQRRGQAPRLLIYGASTRATDTPDRFRGSGSGTDFILTISRLEPEDFAVYYCQYYDDSPFTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: D
Mutation: NULL
| >6XZW_D|Chain A[auth D]|Lipoprot_C domain-containing protein|Neisseria meningitidis serogroup B (strain MC58) (122586) MVAADIGAGLADALTAPLDHKDKGLQSLTLDQSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLESGEFQVYKQSHSALTAFQTEQIQDSEHSGKMVAKRQFRIGDIAGEHTSFDKLPEGGRATYRGTAFGSDDAGGKLTYTIDFAAKQGNGKIEHLKSPELNVDLAAADIKPDGKRHAVISGSVLYNQAEKGSYSLGIFGGKAQEVAGSAEVKTVNGIRHIGLAAKQEH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: PRO32 PHE33 TYR50 GLY51 SER53 THR54 ARG55 THR57 SER66 GLY67 SER68 TYR92 ASP93 H: PRO28 SER30 SER31 TYR32 TYR33 ASN52 ASN53 SER54 ASN99 ARG100 ARG102 ASP104 D: PHE141 ASP142 LEU144 GLU146 GLY147 ARG149 THR169 ASP171 ALA173 ALA174 LYS175 GLN176 ASN178 ALA194 ALA195 ALA196 ASP197 ILE198 LYS199 PRO200 ARG204 LEU213 GLN216 GLU218 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
