Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 324 |
| PDBID: | 2Q8A |
| Chains: | HL_A |
| Organism: | Plasmodium falciparum 3D7, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.40 |
| Reference: | 10.1371/journal.ppat.0030138 |
| Antibody | |
| Antibody: | 1F9 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Beta-lactoglobulin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >2Q8A_H|Chain C[auth H]|1F9 heavy chain|Mus musculus (10090) EVQLQQSGAELLKPGASVKLSCIVSGFKIKDTSMHWVKQRPEQGLEWIGRIDPANDNSEYDPKFQGKATITADTSSNTAYLQLSSLTSEDTAVYYCTLSHFWGQGTTLTVSSAKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLE |
Light Chain: L
Mutation: NULL
| >2Q8A_L|Chain B[auth L]|1F9 light chain|Mus musculus (10090) SIVMTQTPKFLPVSAGDRVTIICKASQSVSNDVVWYQQKPGQSPKLLIYYASIRYTGVPDRFTGSGYGTDFTFTISTVQVEDLAVYFCQQGFSSPRTFGGGTKLEINRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >2Q8A_A|Chain A|Apical membrane antigen 1|Plasmodium falciparum (36329) GNYMGNPWTEYMAKYDIEEVHGSGIRVDLGEDAEVAGTQYRLPSGKCPVFGKGIIIENSNTTFLTPVATGNQYLKDGGFAFPPTEPLMSPMTLDEMRHFYKDNKYVKNLDELTLCSRHAGNMIPDNDKNSNYKYPAVYDDKDKKCHILYIAAQENNGPRYCNKDESKRNSMFCFRPAKDISFQNYTYLSKNVVDNWEKVCPRKNLQNAKFGLWVDGNCEDIPHVNEFPAIDLFECNKLVFELSASDQPKQYEQHLTDYEKIKEGFKNKNASMIKSAFLPTGAFKADRYKSHGKGYNWGNYNTETQKCEIFNVKPTCLINNSSYIATTALSHPIEVE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ASP32 VAL34 TYR49 TYR50 ILE53 ARG54 TYR55 THR56 GLY91 ARG96 H: GLU1 VAL2 GLN3 LEU4 SER25 GLY26 PHE27 LYS28 LYS30 ASP31 THR32 SER33 HIS35 ARG50 ASP52 ALA54 SER75 ASN77 LEU98 SER99 HIS100 PHE101 A: PHE183 THR186 GLU187 PRO188 LEU189 MET190 PRO192 MET193 THR194 ASP196 GLU197 ARG199 HIS200 PHE201 TYR202 LYS203 ASP204 ASN205 VAL208 LYS209 ARG219 GLY222 ASN223 MET224 ILE225 PRO226 ASN228 LYS230 SER232 TYR234 LYS235 LYS246 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
