Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3178 |
| PDBID: | 6X58 |
| Chains: | AB_E |
| Organism: | Homo sapiens, Human immunodeficiency virus, Escherichia coli |
| Method: | XRD |
| Resolution (Å): | 3.26 |
| Reference: | 10.1016/j.jmb.2021.166909 |
| Antibody | |
| Antibody: | 10E8v4 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | membrane-proximal external region (MPER) of the gp41-transmembrane subunit of the HIV envelope protein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >6X58_A|Chain A, E[auth C]|10E8v4 Fab Heavy Chain|Homo sapiens (9606) EVRLVESGGGLVKPGGSLRLSCSASGFDFDNAWMTWVRQPPGKGLEWVGRITGPGEGWSVDYAESVKGRFTISRDNTKNTLYLEMNNVRTEDTGYYFCARTGKYYDFWSGYPPGEEYFQDWGQGTLVIVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKS |
Light Chain: B
Mutation: NULL
| >6X58_B|Chain B, F[auth D]|10E8v4 Fab Light Chain|Homo sapiens (9606) SELTQDPAVSVALKQTVTITCRGDSLRSHYASWYQKKPGQAPVLLFYGKNNRPSGIPDRFSGSASGNRASLTITGAQAEDEADYYCSSRDKSGSRLSVFGGGTKLTVLSQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPT |
Antigen
Chain: E
Mutation: NULL
| >6X58_E|Chain C[auth E], D[auth F]|gp41 MPER peptide,Putative fluoride ion transporter CrcB|Human immunodeficiency virus (12721) LWNWFDITNWLWYIKSLFAVIIGGSVGCTLRWLLSTKFNSLFPNLPPGTLVVNLLAGLIIGTMLAYFLRQPHLDPFWKLMITTGLCGGLSTFSTFSVEVFALLQAGNYIWALTSVLVHVIGSLIMTALGFFIITILFA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: ASN31 TRP33 THR52 PRO54 GLY55 GLU56 SER59 LYS103 TYR104 TYR105 PHE107 TRP108 GLY110 TYR111 PRO112 PRO113 GLY114 GLU116 B: ARG28 GLY67 ARG90 ARG96 E: LEU-11 TRP-10 ASN-9 TRP-8 PHE-7 THR-4 ASN-3 LEU-1 TRP0 ILE2 LYS3 PHE6 HIS60 PHE64 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
