Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3176 |
| PDBID: | 6X4T |
| Chains: | BE_C |
| Organism: | Homo sapiens, Orectolobus maculatus |
| Method: | XRD |
| Resolution (Å): | 3.15 |
| Reference: | 10.1038/s41467-020-18828-4 |
| Antibody | |
| Antibody: | Prezalumab Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Prezalumab(Phase-II); Rozibafusp(Phase-II); |
| Antigen | |
| Antigen: | ICOS ligand |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >6X4T_B|Chain B, D|Prezalumab Fab Heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFTFSSYWMSWVRQAPGKGLEWVAYIKQDGNEKYYVDSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCAREGILWFGDLPTFWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: E
Mutation: NULL
| >6X4T_E|Chain E, F|Prezalumab Fab Light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQGISNWLAWYQQKPEKAPKSLIYAASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYDSYPRTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >6X4T_C|Chain A, C|ICOS ligand|Homo sapiens (9606) TGDTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNRALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSVEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWINKTDNSLLDQALQNDTVFLNMRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVSGTENLYFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLU1 VAL2 GLY26 PHE27 THR28 SER30 SER31 TYR32 GLN52 ASP53 ASN73 ARG94 ILE97 LEU98 TRP99 PRO100 E: ASN31 TRP32 TYR49 ALA50 SER53 GLN55 SER56 C: TYR51 TYR53 GLN55 GLU58 SER59 LYS60 VAL62 TYR65 ILE67 GLN69 ASN70 SER72 GLU74 ASN75 VAL76 HIS112 LEU114 LEU116 GLN118 GLY121 PHE122 GLN123 GLU124 SER127 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
