Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3175 |
| PDBID: | 6X07 |
| Chains: | B_A |
| Organism: | Saccharomyces cerevisiae S288C, Vicugna pacos |
| Method: | XRD |
| Resolution (Å): | 2.10 |
| Reference: | 10.1038/s41467-020-19884-6 |
| Antibody | |
| Antibody: | SAN12 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Nucleoporin NIC96 from S. cerevisiae |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >6X07_B|Chain B|VHH-SAN12|Vicugna pacos (30538) MQVQLVETGGGLVQAGGSLRLSCATSGFNFRLRTMGWYRQAPGKERELVASITSGGSTDYADSVKGRFTISRDNAKNTISLEMNSLKPDDTAVYYCNIWAPTTAAITNWGQGTQVTVSSLPETGGLEHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >6X07_A|Chain A|Nucleoporin NIC96|Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (559292) GPGSEFELGAPAGRQGNKGNNILNSNESRLNVNENNILREKFENYARIVFQFNNSRQANGNFDIANEFISILSSANGTRNAQLLESWKILESMKSKDINIVEVGKQYLEQQFLQYTDNLYKKNMNEGLATNVNKIKSFIDTKLKKADKSWKISNLTVINGVPIWALIFYLLRAGLIKEALQVLVENKANIKKVEQSFLTYFKAYASSKDHGLPVEYSTKLHTEYNQHIKSSLDGDPYRLAVYKLIGRCDLSRKNIPAVTLSIEDWLWMHLMLIKEKDAENDPVYERYSLEDFQNIIISYGPSRFSNYYLQTLLLSGLYGLAIDYTYTFSEMDAVHLAIGLASLKLFKIDSSTRLTKKPKRDIRFANILANYTKSFRYSDPRVAVEYLVLITLNEGPTDVELCHEALRELVLETKEFTVLLGKIGRDGARIPGVIEERQPLLHVRDEKEFLHTITEQAARRADEDGRIYDSILLYQLAEEYDIVITLVNSLLSDTLSASDLDQPLVGPDDNSETNPVLLARRMASIYFDNAGISRQIHVKNKEICMLLLNISSIRELYFNKQWQETLSQMELLDLLPFSDELSARKKAQDFSNLDDNIVKNIPNLLIITLSCISNMIHILNESKYQSSTKGQQIDSLKNVARQCMIYAGMIQYRMPRETYSTLINIDVSL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: PHE27 ASN28 LEU31 ARG32 THR33 SER50 THR52 SER53 GLY54 GLY55 SER56 THR57 ASP58 ASN73 TRP98 PRO100 THR101 A: ARG629 ASP632 TYR644 GLU649 ASP651 ILE652 THR655 LEU656 ARG704 GLN705 HIS707 VAL708 LYS709 ASN710 ILE713 ASN762 ASP764 ASP765 ASN766 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
