Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3171 |
| PDBID: | 6WZT |
| Chains: | ED_B |
| Organism: | Influenza A virus (A/reassortant/NYMC X-217(A/Puerto Rico/8/1934 x A/Victoria/361/2011)(H3N2)), Macaca fascicularis |
| Method: | EM |
| Resolution (Å): | 4.70 |
| Reference: | 10.1126/scitranslmed.abe5449 |
| Antibody | |
| Antibody: | 3B10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H3N2 hemagglutinin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >6WZT_E|Chain B[auth E], E[auth G], H|Cyno antibody heavy chain|Macaca fascicularis (9541) QVQLQESGPGLVKPSETLSLTCAVSGGTFSAYYWGWIRQPPGKGLEWIGSISGGSGSTDYSPSLKSRATISRDTSKNQFSLKLTSVTAADTAVYYCVRKYWGDYYANWFDVWGPGVLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGSLTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYVCNVNHKPSNTKVDKRVEIKTCGGGSKPPT |
Light Chain: D
Mutation: NULL
| >6WZT_D|Chain C[auth D], F, I[auth L]|Cyno antibody light chain|Macaca fascicularis (9541) DIQMTQSPSSLSASVGDTVTITCRASQSISSWLAWYQQKPGKAPKLLIYKASSLQGGVPSRFSGSGSGSDFTLTISSLQSEDFATYYCQQYSGRPPTFGQGTKVEIKRAVAAPSVFIFPPSEDQVKSGTVSVVCLLNNFYPREASVKWKVDGALKTGNSQESVTEQDSKDNTYSLSSTLTLSSTDYQSHNVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >6WZT_B|Chain A, D[auth B], G[auth C]|Hemagglutinin|Influenza A virus (1507389) QKLPGNDNSTATLCLGHHAVPNGTIVKTITNDQIEVTNATELVQNSSIGEICDSPHQILDGENCTLIDALLGDPQCDGFQNKKWDLFVERSKAYSNCFPYDVPDYASLRSLVASSGTLEFNNESFNWTGVTQNGTSSACIRRSNNSFFSRLNWLTQLNFKYPALNVTMPNNEQFDKLYIWGVHHPVTDKDQIFLYAQSSGRITVSTKRSQQAVIPNIGYRPRIRNIPSRISIYWTIVKPGDILLINSTGNLIAPRGYFKIRSGKSSIMRSDAPIGKCNSECITPNGSIPNDKPFQNVNRITYGACPRYVKQSTLKLATGMRNVPEKQTRGIFGAIAGFIENGWEGMVDGWYGFRHQNSEGRGQAADLKSTQAAIDQINGKLNRLIGKTNEKFHQIEKEFSEVEGRIQDLEKYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFEKTKKQLRENAEDMGNGCFKIYHKCDNACIGSIRNGTYDHDVYRDEALNNRFQIK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: VAL2 GLY27 THR28 SER30 ALA31 TYR32 TYR33 SER52 GLY54 SER55 THR74 ARG98 TYR100 TRP101 GLY102 ASP103 TYR104 TYR105 ASN107 ASP110 D: TYR49 LYS50 B: PRO21 GLU325 LYS326 GLN327 THR328 ARG329 ILE331 ALA334 ILE335 GLU344 GLY345 MET346 VAL347 ASP348 ARG354 HIS355 GLU359 GLY360 ARG361 GLY362 GLN363 ALA364 ALA365 LEU367 ASN475 ILE478 ARG482 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
