Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3168 |
| PDBID: | 6WW5 |
| Chains: | CD_B |
| Organism: | Vibrio cholerae, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.15 |
| Reference: | 10.7554/eLife.61350 |
| Antibody | |
| Antibody: | 84 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | DASS family sodium-coupled anion symporter |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >6WW5_C|Chain C, D[auth E]|Fab84 Heavy Chain|Homo sapiens (9606) MKKNIAFLLASMFVFSIATNAYAEISEVQLVESGGGLVQPGGSLRLSCAASGFTIYSSSIHWVRQAPGKGLEWVASIYSSSGSTSYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARYNIHYWRWYNPAGQAMDYWGQGTLVTVFNQIKGGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: D
Mutation: NULL
| >6WW5_D|Chain E[auth D], F|Fab84 Light Chain|Homo sapiens (9606) MKKNIAFLLASMFVFSIATNAYASDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQRYGLLVTFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >6WW5_B|Chain A, B|DASS family sodium-coupled anion symporter|Vibrio cholerae (666) REWFLHRNSLIVLADVALFLALYHFLPFEHNVVLGISMLAFIAVLWLTEALHVTVTAILVPVMAVFFGIFETQAALNNFANSIIFLFLGGFALAAAMHHQGLDKVIADKVLAMAQGKMSVAVFMLFGVTALLSMWISNTATAAMMLPLVLGVLSKVDADKQRSTYVFVLLGVAYSASIGGIATLVGSPPNAIAAAEVGLSFTDWMKFGLPTAMMMLPMAIAILYFLLKPTLNGMFELDRAPVNWDKGKVVTLGIFGLTVFLWIFSSPINAALGGFKSFDTLVALGAILMLSFARVVHWKEIQKTADWGVLLLFGGGLCLSNVLKQTGTSVFLANALSDMVSHMGIFVVILVVATFVVFLTEFASNTASAALLIPVFATVAEAFGMSPVLLSVLIAVAASCAFMLPVATPPNAIVFASGHIKQSEMMRVGLYLNIACIGLLTAIAMLFWQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: TYR30 SER31 TYR52 SER53 SER54 SER55 GLY56 SER57 THR58 SER59 ILE101 HIS102 TYR103 TRP104 ARG105 TRP106 D: GLN27 SER28 VAL29 SER30 SER31 ALA32 ARG66 SER67 GLY68 THR69 ARG91 TYR92 GLY93 LEU94 LEU95 B: GLU42 ASN44 VAL45 PHE83 ASN94 ALA208 GLU209 VAL210 GLY211 LEU330 SER333 ASN334 LYS337 GLN338 THR339 GLY340 SER342 VAL343 PHE344 ALA346 ASN347 SER350 VAL353 SER354 MET356 GLY357 ILE358 THR391 GLU394 ALA395 PHE396 GLY397 MET398 SER399 TRP461 GLN462 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
