Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3141 |
| PDBID: | 6WOQ |
| Chains: | JK_E |
| Organism: | Homo sapiens, Hepatitis C virus (isolate H) |
| Method: | XRD |
| Resolution (Å): | 3.67 |
| Reference: | 10.1126/sciadv.abb5642 |
| Antibody | |
| Antibody: | HC1AM Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Hepatitis C Virus Envelope glycoprotein E2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: J
Mutation: NULL
| >6WOQ_J|Chain D[auth G], I[auth J]|Fab HC1AM heavy chain|Homo sapiens (9606) QLQLVQSGAEVRKPGSSVKVSCKASGGTYNGEVISWVRQAPGEGLEWLGVFIPMFGIADYAQKFHGRVTISADRTTSTTYMELSSLRSEDTAIYYCAKVLQVGRNLVVRPLDLWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCGS |
Light Chain: K
Mutation: NULL
| >6WOQ_K|Chain E[auth H], J[auth K]|Fab HC1AM light chain|Homo sapiens (9606) EIVLTQSPGILSLSPGERATLSCRASRTISRTHLAWYQQKPGQAPRLLIYGVSSRAIGIPDRFSGSGSGADFTLTISRLEPEDFAVYYCHQYGNSPQTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: E
Mutation: NULL
| >6WOQ_E|Chain C[auth E], H[auth F]|Envelope glycoprotein E2|Hepatitis C virus (isolate H) (11108) ARQLINTNGSWHINSTALNCNESLNTGWLAGLFYQHKFDSSGCPERLASCGSSGCWHYPPRPCGIVPAKSVCGPVYCFTPSPVVVGTTDRSGAPTYSWGANDTDVFVLNNTRPPLGNWFGCTWMNSTGFTKVCGAPPGGPTDGGSGPWITPRCMVDYPYRLWHYPCTINYTIFKVRMYVGGVEHRLEAACN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| J: TRP47 VAL50 MET53 PHE54 ILE56 ALA57 ASP58 TYR59 VAL98 GLY99 K: ARG30 HIS32 GLY92 ASN93 SER94 GLN96 E: TRP420 ILE422 ASN423 LEU427 ASN428 CYS429 ASN430 SER432 LYS500 CYS503 GLY504 PRO505 TYR527 SER528 TRP529 ALA531 ASN532 ASP533 SER557 TYR613 TRP616 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
