Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3139 |
| PDBID: | 6WO5 |
| Chains: | HL_E |
| Organism: | Homo sapiens, Hepatitis C virus (isolate H) |
| Method: | XRD |
| Resolution (Å): | 2.62 |
| Reference: | 10.1126/sciadv.abb5642 |
| Antibody | |
| Antibody: | 212.1.1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Hepatitis C Virus Envelope glycoprotein E2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6WO5_H|Chain E[auth G], G[auth H]|Fab 212.1.1 heavy chain|Homo sapiens (9606) QVQLVQSGAEVKKPGSSVKVSCKASGGTLSSYGISWVRQAPGQGLEWLGGSIPILGTSVYAQKFQGRVTMTADESTSTAHMELSSLRFDDTAIYYCAGVREGMAAISGKNAFDIWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCGS |
Light Chain: L
Mutation: NULL
| >6WO5_L|Chain F[auth I], H[auth L]|Fab 212.1.1 light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWYQQKPGKVPKLLIYAASTLQSGVPSRFSGSGYGTEFTLTISSLQPEDVATYYCQQHDNLPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: E
Mutation: NULL
| >6WO5_E|Chain I[auth E], J[auth F]|Envelope glycoprotein E2|Hepatitis C virus (isolate H) (11108) ARQLINTNGSWHINSTALNCNESLNTGWLAGLFYQHKFDSSGCPERLASCGSSGCWHYPPRPCGIVPAKSVCGPVYCFTPSPVVVGTTDRSGAPTYSWGANDTDVFVLNNTRPPLGNWFGCTWMNSTGFTKVCGAPPGGPTDGGSGPWITPRCMVDYPYRLWHYPCTINYTIFKVRMYVGGVEHRLEAACN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER30 SER31 PRO52 ILE53 LEU54 GLY55 THR56 VAL58 GLN61 GLN64 GLY98 MET99 L: TYR32 TYR49 ALA50 HIS91 ASN93 LEU94 E: ILE422 ASN423 THR425 LEU427 ASN428 CYS429 ASN430 GLU431 LEU433 ASN434 THR435 GLY436 TRP437 LEU438 CYS503 GLY504 PRO505 TRP529 ALA531 MET555 TYR613 TRP616 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
