Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3138 |
| PDBID: | 6WO4 |
| Chains: | HL_E |
| Organism: | Homo sapiens, Recombinant Hepatitis C virus HK6a/JFH-1 |
| Method: | XRD |
| Resolution (Å): | 2.35 |
| Reference: | 10.1126/sciadv.abb5642 |
| Antibody | |
| Antibody: | COVA2-04 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Hepatitis C Virus Envelope Glycoprotein E2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6WO4_H|Chain A[auth H]|Fab HC11 heavy chain|Homo sapiens (9606) EVQLVESGAEVKKPGSSVKVSCKASGATFSSFIFSWVRQAPGQGPEWMGGIIPMFGTAKYAPRLQGRVTITADESTSTAYMDLSSLRFEDTAVYYCAMEVPGFCRGGSCSGYMDVWGKGTLVTVSPASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCGS |
Light Chain: L
Mutation: NULL
| >6WO4_L|Chain C[auth L]|Fab HC11 light chain|Homo sapiens (9606) GVPTQVLGLLLLWLTDARCQAAVLTQSPGTLSLSPGERATLSCRASHSVSSSNLAWYQQKPGQAPRLLMYGASRRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPITFGQGTRLDIKGTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: E
Mutation: NULL
| >6WO4_E|Chain B[auth E]|Envelope glycoprotein E2|Recombinant Hepatitis C virus HK6a/JFH-1 (595609) QLINTNGSWHINRTALNCNDSLQTGFITSLFYAKNVDSSGCPERMAACGSSGCWHYAPRPCDVVSARTVCGPVYCFTPSPVVVGTTDKLGIPTYNWGENETDVFMLESLRPPTGGWFGCTWMNSTGFTKTCGAPPGGPTDGGSGPWITPRCLVDYPYRLWHYPCTVNFTLHKVRMFVGGIEHRFDAACN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ALA27 THR28 SER31 PHE32 ILE33 MET53 PHE54 THR56 MET94 GLU95 VAL96 PRO97 GLY98 PHE99 L: SER30 SER31 ASN32 TYR49 GLY50 ARG53 TYR91 E: ILE422 LEU427 ASN428 CYS429 ASN430 ASP431 SER432 LEU433 GLN434 ILE438 THR439 LEU441 PHE442 TYR443 ALA444 LYS445 TRP529 GLU531 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
