Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3132 |
| PDBID: | 6WMW |
| Chains: | MN_B |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.91 |
| Reference: | 10.1038/s41591-020-0945-x |
| Antibody | |
| Antibody: | 25M22 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | GDNF family receptor alpha-like (GFRAL) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: M
Mutation: NULL
| >6WMW_M|Chain D[auth M]|FAB25M22 heavy chain fragment|Homo sapiens (9606) QVQLQQSGPDLVKPGASVKISCKASGYTFTSYWVNWMKQRPGKGLEWIGRIYPGDGDTNYNGKFKGKATLTADKSSSTAYMQLSSLTSEDSAVYFCARAYLLRLRRTGYYAMDYWGQGTSVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDEVD |
Light Chain: N
Mutation: NULL
| >6WMW_N|Chain E[auth N]|FAB25M22 light chain|Homo sapiens (9606) MDMRVPAQLLGLLLLWLRGARCDVVLTQTPLSLPVNIGDQASISCKSTKSLLNSDEFTYLDWYLQKPGQSPQLLIFLVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYYCFQSNYLPYTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >6WMW_B|Chain A[auth B]|GDNF family receptor alpha-like|Homo sapiens (9606) HHHHHHGGWNLTTRSHHGFKGMWSCLEVAEACVGDVVCNAQLASYLKACSANGNPCDLKQCQAAIRFFYQNIPFNIAQMLAFCDCAQSDIPCQQSKEALHSKTCAVNMVPPPTCLSVIRSCQNDELCRRHYRTFQSKCWQRVTRKCHEDENCISTLSKQDLTCSGSDDCKAAYIDILGTVLQVQCTCRTITQSEESLCKIFQHMLHRKSCFNYPTLSNVKGMALYTRKHANKITLTGFHSPFNGE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| M: VAL2 GLY26 TYR27 THR28 THR30 SER31 TYR32 TRP33 TYR52 ASP55 ASP57 TYR100 LEU101 LEU102 ARG103 LEU104 ARG105 ARG106 THR107 GLY108 TYR110 N: GLU56 B: LEU132 ALA135 GLU136 VAL139 VAL142 ASN145 ALA146 GLN147 LEU148 ALA149 SER150 LEU152 LYS153 SER156 ALA157 GLN166 ILE196 PRO197 GLN200 SER201 GLU203 ALA204 LEU205 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
