Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3131 |
| PDBID: | 6WMW |
| Chains: | HL_B |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.91 |
| Reference: | 10.1038/s41591-020-0945-x |
| Antibody | |
| Antibody: | 3P10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | GDNF family receptor alpha-like (GFRAL) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6WMW_H|Chain B[auth H]|FAB3P10 heavy chain fragment|Homo sapiens (9606) QIQLVQSGPELKKPGETVKISCKASGYTFTDYGVIWVKQAPGKALKWMGWINTYTGEPTYADDLKGRFAFSLETSASSASLQINNLKNEDTATYFCARRYGPEDIDYWGQGTTLTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDEVD |
Light Chain: L
Mutation: NULL
| >6WMW_L|Chain C[auth L]|FAB3P10 light chain|Homo sapiens (9606) DIVLTQSPVSLAVSLGQRATISCRASESVDNYGISFMSWFQQKPGQPPKLLIYAASHQGSGVPARFSGSGSGTDFSLNIHPMEEDDSAMYFCLQSKEVPWTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >6WMW_B|Chain A[auth B]|GDNF family receptor alpha-like|Homo sapiens (9606) HHHHHHGGWNLTTRSHHGFKGMWSCLEVAEACVGDVVCNAQLASYLKACSANGNPCDLKQCQAAIRFFYQNIPFNIAQMLAFCDCAQSDIPCQQSKEALHSKTCAVNMVPPPTCLSVIRSCQNDELCRRHYRTFQSKCWQRVTRKCHEDENCISTLSKQDLTCSGSDDCKAAYIDILGTVLQVQCTCRTITQSEESLCKIFQHMLHRKSCFNYPTLSNVKGMALYTRKHANKITLTGFHSPFNGE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR30 ASP31 TYR32 GLY33 TRP50 ASN52 THR53 TYR54 ARG99 TYR100 GLY101 PRO102 GLU103 ASP104 ILE105 ASP106 L: TYR32 GLY33 ILE34 PHE36 LEU50 TYR53 ALA54 HIS57 GLY59 THR73 B: GLN193 ASN213 MET214 VAL215 PRO216 PRO217 GLN290 CYS291 THR292 CYS293 ARG294 THR295 ILE296 THR297 GLN298 SER299 GLU301 LYS305 GLN308 HIS309 HIS312 SER315 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
