Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3124 |
| PDBID: | 6WIT |
| Chains: | AB_C |
| Organism: | Macaca mulatta, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.79 |
| Reference: | 10.1371/journal.ppat.1009543 |
| Antibody | |
| Antibody: | NHP-D15.SD7 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 clade C 16055 strain V1V2 1FD6 scaffold |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >6WIT_A|Chain A, D[auth H]|NHP GN1-SD7 Fab Heavy Chain|Macaca mulatta (9544) EVQLVQSGAEVKKPGASMKISCKASGYTFTDYSLHWVRQTPGKGLEWMGRVDPEDGESHYAQKFQDRVTITADTSTDTAYMEVRSLRSEDTAMYYCATDHREDGPTYFSGVQWVPFRFWGQGVLVTVSSASTKGPSVFPLAPSXXXXXXGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC |
Light Chain: B
Mutation: NULL
| >6WIT_B|Chain B, E[auth L]|NHP GN1-SD7 Fab Light Chain|Macaca mulatta (9544) VVFTQPHSVSGSPGQTVTISCTRSSGSIDSEYVQWYQQRPGSAPTTLIYKDNQRPSGVPDRFSGSIDSSSNSASLAISGLKSEDEADYYCQSPDGRYNRVFGGGTRLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPT |
Antigen
Chain: C
Mutation: NULL
| >6WIT_C|Chain C, F[auth I]|16055 V1V2 1FD6 Scaffold|Homo sapiens (9606) MTTFKLAACVTLECRQVXXXXXXXXXXXXXXXXXXEIKNCSFNATTXXXDKKQKVYALFYRLDIVPLEEERKGNSSKYRLINCQTTTTEAVDAATAAKVFKQYANDNGIDGEWTYDDATKTFTVTEGLE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: THR30 ASP31 PRO52 GLU53 ASP54 THR73 ARG97 ASP99 B: ILE28 ASP29 SER30 GLU31 TYR32 GLN34 LYS50 ASP51 PRO91 GLY93 ARG94 ARG96 C: MET118 THR119 THR120 LYS122 GLN133 GLU153 LYS155 TYR177 ARG178 LEU179 ILE181 VAL182 PRO183 LEU184 GLU185 GLY186 ARG192 ILE194 GLU202 ALA231 THR232 LYS233 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
