Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3116 |
| PDBID: | 6WGL |
| Chains: | AB_C |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.82 |
| Reference: | 10.1371/journal.pone.0232311 |
| Antibody | |
| Antibody: | Dupilumab Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Dupilumab(Approved) |
| Antigen | |
| Antigen: | Interleukin-4 receptor subunit alpha |
| Antigen mutation: | No |
| Durg Target: | P24394 |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >6WGL_A|Chain A|Dupilumab Fab heavy chain|Homo sapiens (9606) EVQLVESGGGLEQPGGSLRLSCAGSGFTFRDYAMTWVRQAPGKGLEWVSSISGSGGNTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKDRLSITIRPRYYGLDVWGQGTTVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYGHHHHHH |
Light Chain: B
Mutation: NULL
| >6WGL_B|Chain B|Dupilumab Fab light chain|Homo sapiens (9606) DIVMTQSPLSLPVTPGEPASISCRSSQSLLYSIGYNYLDWYLQKSGQSPQLLIYLGSNRASGVPDRFSGSGSGTDFTLKISRVEAEDVGFYYCMQALQTPYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTQGTTSVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >6WGL_C|Chain C|Interleukin-4 receptor subunit alpha|Homo sapiens (9606) MKVLQEPTCVSDYMSISTCEWKMNGPTNCSTELRLLYQLVFLLSEAHTCIPENNGGAGCVCHLLMDDVVSADNYTLDLWAGQQLLWKGSFKPSEHVKPRAPGNLTVHTNVSDTLLLTWSNPYPPDNYLYNHLTYAVNIWSENDPADFRIYNVTYLEPSLRIAASTLKSGISYRARVRAWAQLYNTTWSEWSPSTKWHNSYREPFEQHGGGGSHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: ARG30 ALA33 SER50 ILE51 SER52 SER54 ASN57 TYR59 ASP99 ARG100 SER102 ILE103 THR104 ILE105 ARG106 PRO107 ARG108 TYR109 B: TYR31 SER32 TYR35 TYR37 LEU97 GLN98 THR99 TYR101 C: TYR37 GLN38 LEU39 VAL40 PHE41 LEU42 LEU43 SER44 GLU45 HIS47 LEU64 ASP66 ASP67 VAL69 ALA71 ASP72 TYR74 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
