Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3108 |
| PDBID: | 6WAS |
| Chains: | AB_G |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.90 |
| Reference: | 10.1371/journal.ppat.1009543 |
| Antibody | |
| Antibody: | D19.PA8 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 clade C 16055 strain V1V2 1FD6 scaffold |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >6WAS_A|Chain A, C[auth H]|GN1_PA8 Fab Heavy chain|Homo sapiens (9606) QVQLVESGGGLAKPGGSLRLSCAASGITFSEDYMHWVRQASGKGLEWVSRISYDSDNTWYADSVKGRFTISRENAKNTLYLQMDSLRAEDTAVYYCARAPVWTGYTSLDVWGRGVLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKS |
Light Chain: B
Mutation: NULL
| >6WAS_B|Chain B, D[auth L]|GN1_PA8 Fab Light chain|Homo sapiens (9606) QVVFSQPHSVSGSPGQTVTISCTRSSGSIDNEYVRWYQQRPGSVPTIVIYKDNQRPSGVPDRFSGSIDSSSNSASLAISGLQSEDEADYYCQSSDDNFNWVFGGGTRLTVLRQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: G
Mutation: NULL
| >6WAS_G|Chain E[auth G], F[auth J]|1FD6 16055 V1V2 scaffold|Homo sapiens (9606) MTTFKLAACVTLECRQVNTTNATSSVNVTNGEEIKNCSFNATTEIRDKKQKVYALFYRLDIVPLEEERKGNSSKYRLINCQTTTTEAVDAATAAKVFKQYANDNGIDGEWTYDDATKTFTVTEGLEVLFQGPGHHHHHHHHSAWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: TYR33 ARG50 TYR52 VAL97 TRP98 B: ASP29 ASN30 GLU31 TYR32 ARG34 LYS50 ASP51 SER91 TRP96 G: GLN133 GLU153 LYS155 ARG178 VAL182 PRO183 LEU184 GLU185 ASN187 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
