Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3094 |
| PDBID: | 6W4S |
| Chains: | HL_F |
| Organism: | Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.20 |
| Reference: | 10.1038/s41586-020-2668-z |
| Antibody | |
| Antibody: | 45D8 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Solute carrier family 40 member 1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6W4S_H|Chain B[auth H]|Fab45D8 Heavy Chain|Mus musculus (10090) EVQLQESGPGLAKPSQTLSLTCSVTGSSITSDYWNWIRKFPGNKLEYMGYISYSGSTYYNPSLKSQISITRDTSKNHYYLQLNSVTTEDTATYYCARQGLRNWYFDVWGTGTTVTVSSAKTTAPSVYPLAPVCGGTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPALLQSGLYTLSSSVTVTSNTWPSQTITCNVAHPASSTKVDKKIEPRVP |
Light Chain: L
Mutation: NULL
| >6W4S_L|Chain C[auth L]|Fab45D8 Light Chain|Mus musculus (10090) DIVLTQSPASLPVSLGQRATISCRASKSVSASAYSYMHWYQQKPGQPPKPLIYLASNLESGVPARFSGSGSGTDFTLNIHPVEEEDAATYYCQHNRELPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: F
Mutation: NULL
| >6W4S_F|Chain A[auth F]|Solute carrier family 40 member 1|Homo sapiens (9606) MTRAGDHNRQRGCCGSLADYLTSAKFLLYLGHSLSTWGDRMWHFAVSVFLVELYGNSLLLTAVYGLVVAGSVLVLGAIIGDWVDKNARLKVAQTSLVVQNVSVILCGIILMMVFLHKHELLTMYHGWVLTSCYILIITIANIANLASTATAITIQRDWIVVVAGEDRSKLANMNATIRRIDQLTNILAPMAVGQIMTFGSPVIGCGFISGWNLVSMCVEYVLLWKVYQKTPALAVKAGLKEEETELKQLNLHKDTEPKPLEGTHLMGVKDSNIHELEHEQEPTCASQMAEPFRTFRDGWVSYYNQPVFLAGMGLAFLYMTVLGFDCITTGYAYTQGLSGSILSILMGASAITGIMGTVAFTWLRRKCGLVRTGLISGLAQLSCLILCVISVFMPGSPLDLSVSPFEDIRSRFIQGESITPTKIPEITTEIYMSNGSNSANIVPETSPESVPIISVSLLFAGVIAARIGLWSFDLTVTQLLQENVIESERGIINGVQNSMNYLLDLLHFIMVILAPNPEAFGLLVLISVSFVAMGHIMYFRFAQNTLGNKLFACGPDAKEVRKENQANTSVVGSGLEVLFQGPGAAEDQVDPRLIDGKHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TYR33 TYR50 TYR53 LEU100 ARG101 ASN102 TRP103 L: ALA31 SER32 ALA33 TYR34 TYR36 LEU54 ASN95 ARG96 GLU97 LEU98 TYR100 F: VAL51 GLU52 LEU53 TYR54 GLY55 LEU115 HIS116 LYS117 HIS118 GLU119 LEU121 THR122 MET123 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
