Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3086 |
| PDBID: | 6W1C |
| Chains: | IM_F |
| Organism: | Mayaro virus (strain Brazil), Homo sapiens |
| Method: | EM |
| Resolution (Å): | 5.30 |
| Reference: | 10.1016/j.chom.2020.07.008 |
| Antibody | |
| Antibody: | CHK-265 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Mayaro virus E2 glycoprotein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >6W1C_I|Chain I, K[auth J], M[auth K], O[auth L]|Fab CHK-265 heavy chain|Homo sapiens (9606) QIQLVQSGREVKNPGETVKISCKASGYTFTEYPMLWVKQAPGKGFRWMGLIYTNTGEPTYAEEFKGRFVFSLEISASTAYLQINNLTNEDTATYFCVRDYFISLDYWGQGTTLTVSSAKTTAPSVYPLAPVCGGTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPALLQSGLYTLSSSVTVTSNTWPSQTITCNVAHPASSTKVDKKIESRR |
Light Chain: M
Mutation: NULL
| >6W1C_M|Chain J[auth M], L[auth N], N[auth O], P|Fab CHK-265 light chain|Homo sapiens (9606) QAVVTQESALTTSPGETVTLTCRSNIGAVTSSNCANWVQEKPDHFFTGLIGDTNNRRSGVPARFSGSLIGDKAALTITGAQTEDEAIYFCALWYNNLWVFGGGTKLTVLGQPKSSPSVTLFPPSSEELETNKATLVCTITDFYPGVVTVDWKVDGTPVTQGMETTQPSKQSNNKYMASSYLTLTARAWERHSSYSCQVTHEGHTVEKSLSR |
Antigen
Chain: F
Mutation: NULL
| >6W1C_F|Chain E, F, G, H|E2 glycoprotein|Mayaro virus (strain Brazil) (374990) STANHFNAYKLTRPYVAYCADCGMGHSCHSPAMIENIQADATDGTLKIQFASQIGLTKTDTHDHTKIRYAEGHDIAEAARSTLKVHSSSECTVTGTMGHFILAKCPPGERISVSFVDSKNEHRTCRIAYHHEQRLIGRERFTVRPHHGIELPCTTYQLTTAETSEEIDMHMPPDIPDRTILSQQSGNVKITVNGRTVRYSSSCGSQAVGTTTTDKTINSCTVDKCQAYVTSHTKWQFNSPFVPRRMQAERKGKVHIPFPLINTTCRVPLAPEALVRSGKREATLSLHPIHPTLLSYRTFGAERVFDEQWITAQTEVTIPVPVEGVEYQWGNHKPQRFVVA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: GLU31 TYR52 ASN54 THR55 GLU57 ASP99 TYR100 PHE101 ILE102 M: SER249 SER250 ASN313 ASN314 F: SER182 GLN183 GLN184 SER185 GLY186 ASN187 VAL188 LYS189 SER202 CYS220 THR221 VAL222 ASP223 LYS224 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
