Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3085 |
| PDBID: | 6W16 |
| Chains: | HL_F |
| Organism: | human metapneumovirus, Human immunodeficiency virus 1, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.10 |
| Reference: | 10.1371/journal.ppat.1008942 |
| Antibody | |
| Antibody: | 458 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human metapneumovirus (hMPV) fusion (F) protein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6W16_H|Chain B[auth H]|458 Fab heavy chain|Homo sapiens (9606) QGKLVESGGGVIQPGRSLRLSCAASGFDFSRYGLHWVRQAPGKGLEWVAVIVYAGSNKYYADSVKGRFTISKDNSKNTMHLQMSDLRTEDTAVYYCARDQAFDLWGQGTMVTVSSASTKGPSVFPLAPSSKSTGGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD |
Light Chain: L
Mutation: NULL
| >6W16_L|Chain C[auth L]|458 Fab light chain|Homo sapiens (9606) DIQMTQSPASLSASVGDRVTITCQASQGISRSVNWYQQKPGKAPKLLIFDASHLERGVPSRFSGSGYGTDFTFTISSLQPEDIATYYCQQYDNLRISFGQGTRLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: F
Mutation: NULL
| >6W16_F|Chain A[auth F]|Fusion glycoprotein F0,Envelope glycoprotein fusion|Human metapneumovirus (162145) MSWKVMIIISLLITPQHGLKESYLEESCSTITEGYLSVLRTGWYTNVFTLEVGDVENLTCTDGPSLIKTELDLTKSALRELKTVSADQLAREEQIENPRQSKKRKRRVATAAAVTAGIAIAKTIRLESEVNAIKGALKTTNEAVSTLGNGVRVLATAVRELKEFVSKNLTSAINKNKCDIADLKMAVSFSQFNRRFLNVVRQFSDNAGITPAISLDLMTDAELARAVSYMPTSAGQIKLMLENRAMVRRKGFGILIGVYGSSVIYMVQLPIFGVIDTPCWIIKAAPSCSEKDGNYACLLREDQGWYCKNAGSTVYYPNEKDCETRGDHVFCDTAAGINVAEQSRECNINISTTNYPCKVSTGRHPISMVALSPLGALVACYKGVSCSIGSNRVGIIKQLPKGCSYITNQDADTVTIDNTVYQLSKVEGEQHVIKGRPVSSSFDPIRFPEDQFNVALDQVFESIENSQALVDQSNKILNSAEKGNTSGRENLYFQGGGGGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGGTEGRHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLN1 GLY27 PHE28 ASP29 ARG36 TYR37 VAL57 TYR58 ALA59 SER63 ASN64 TYR66 ASP107 GLN108 ALA114 PHE115 ASP116 L: SER36 ARG37 SER38 PHE55 ASP56 HIS66 LEU67 ARG69 TYR83 TYR107 ASP108 ASN109 LEU114 ARG115 F: GLU56 ASN57 THR59 CYS60 THR61 PRO64 LYS68 THR69 GLU70 ASP72 LEU73 LYS75 SER76 ALA77 ARG79 GLU80 LYS82 THR83 VAL84 ALA86 ASP87 ASN180 LEU201 ARG205 SER208 ASP209 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
