Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3057 |
| PDBID: | 6VVU |
| Chains: | JK_B |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1038/s41467-020-20143-x |
| Antibody | |
| Antibody: | E104.v1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Tryptase alpha/beta-1 |
| Antigen mutation: | No |
| Durg Target: | Q15661; P20231; |
Sequence information
Antibody
Heavy Chain: J
Mutation: NULL
| >6VVU_J|Chain E, G, H, J|Fab E104.v1 heavy chain|Homo sapiens (9606) EVQLVESGPGLVKPSETLSLTCTVSRFSLIGYAITWIRQPPGKGLEWIGGISSAATTFYSSWAKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCARDPRGYGAALDRLDLWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: K
Mutation: NULL
| >6VVU_K|Chain F, I, K, L|Fab E104.v1 light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCQSIKSVYNNRLGWYQQKPGKAPKLLIYETSILTSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCAGGFDRSGDTTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >6VVU_B|Chain A, B, C, D|Tryptase alpha/beta-1|Homo sapiens (9606) MLNLLLLALPVLASRAYAAPAPGQALQRVGIVGGQEAPRSKWPWQVSLRVHGPYWMHFCGGSLIHPQWVLTAAHCVGPDVKDLAALRVQLREQHLYYQDQLLPVSRIIVHPQFYTAQIGADIALLELEEPVNVSSHVHTVTLPPASETFPPGMPCWVTGWGDVDNDERLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIVRDDMLCAGNTRRDSCQGDSGGPLVCKVNGTWLQAGVVSWGEGCAQPNRPGIYTRVTYYLDWIHHYVPKKP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| J: SER28 ILE30 GLY31 TYR32 SER52 SER53 ALA54 ALA55 THR56 PHE58 PRO96 ARG97 GLY98 TYR99 K: TYR29 ASN30 ARG32 TYR49 GLU50 ARG94 B: HIS36 TYR37 TRP38 GLN50 ASP60 LEU61 ALA62 ARG65 ASP80 GLN81 LEU82 LEU83 PRO84 VAL85 SER86 ARG87 GLU107 GLU109 GLU110 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
