Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3007 |
| PDBID: | 6VI4 |
| Chains: | C_B |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 3.30 |
| Reference: | 10.1038/s41467-020-14889-7 |
| Antibody | |
| Antibody: | 6 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Kappa opioid receptor |
| Antigen mutation: | Yes |
| Durg Target: | P41145 |
Sequence information
Antibody
Chain: C
Mutation: NULL
| >6VI4_C|Chain C, D|Nanobody 6|Lama glama (9844) MAQVQLQESGGGLVQAGESLRLSCAASGTIFRLYDMGWYRRVSGNQRELVASITSGGSTKYGDSVKGRFTISRDNAKNTVYLQMSSLKPEDTAVYYCNAEYRTGIWEELLDGWGQGTQVTVSSHHHHHHEPEA |
Antigen
Chain: B
Mutation: I84L
| >6VI4_B|Chain A, B|Kappa opioid receptor|Homo sapiens (9606) GSISPAIPVIITAVYSVVFVVGLVGNSLVMFVIIRYTKMKTATNIYIFNLALADALVTTTMPFQSTVYLMNSWPFGDVLCKIVLSIDYYNMFTSIFTLTMMSVDRYIAVCHPVKALDFRTPLKAKIINICIWLLSSSVGISAIVLGGTKVREDVDVIECSLQFPDDDYSWWDLFMKICVFIFAFVIPVLIIIVCYTLMILRLKSVRLLSGSREKDRNLRRITRLVLVVVAVFVVCWTPIHIFILVEALGSTSHSTAALSSYYFCIALGYTNSSLNPILYAFLDENFKRCFRDFCFPLKMRMERQSTS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: PHE31 ARG32 TYR34 ASP35 GLU100 ARG102 ILE105 TRP106 GLU107 GLU108 LEU109 LEU110 ASP111 B: TYR246 ILE250 LEU253 LYS254 SER255 VAL256 ARG257 LEU258 ARG263 ASP266 ARG267 ARG270 ARG271 THR273 ARG274 LEU277 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
