Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2993 |
| PDBID: | 6VCA |
| Chains: | KJ_C |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.73 |
| Reference: | 10.1074/jbc.RA120.012395 |
| Antibody | |
| Antibody: | TB38 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | 5'-nucleotidase, ecto (CD73), isoform CRA_a |
| Antigen mutation: | No |
| Durg Target: | P21589 |
Sequence information
Antibody
Heavy Chain: K
Mutation: NULL
| >6VCA_K|Chain E, G[auth H], I[auth K], K[auth P]|TB38 heavy chain|Homo sapiens (9606) QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYGMHWVRQAPGKGLEWVAVFWYDGSNKYYADSVKGRFTISRDNSKNTLYLQMNSLSAEDTAVYYCARAPNWDDAFDIWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTHHHHHH |
Light Chain: J
Mutation: NULL
| >6VCA_J|Chain F, H[auth J], J[auth L], L[auth O]|TB38 light chain|Homo sapiens (9606) QTVVTQEPSFSVSPGGTVTLTCGLSSGSVSTSYYPNWYQQTPGQAPRTLIYSTNTRSSGVPDRFSGSILGNKAALTITGAQADDESDYYCVLFMGSGIWVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: C
Mutation: NULL
| >6VCA_C|Chain A, B, C, D|5'-nucleotidase, ecto (CD73), isoform CRA_a|Homo sapiens (9606) WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHADEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIKFSHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition K: GLY26 PHE27 THR28 SER31 TYR32 TRP52 TYR53 ASP54 SER56 ASN57 TYR59 ARG98 PRO100 ASN101 TRP102 ASP103 ASP104 ASP107 ILE108 J: THR31 SER32 TYR34 TYR51 SER52 ASN54 THR55 SER57 SER58 MET94 GLY95 SER96 C: LEU132 LYS133 ALA135 LYS136 PRO138 LYS145 ALA146 LYS147 GLY148 ALA151 SER152 SER155 GLY156 LEU157 TYR158 LEU159 PRO160 TYR161 LYS162 VAL163 PRO165 VAL170 PRO202 GLU203 ASP205 LYS206 THR209 LEU210 ASN211 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
