Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2990 |
| PDBID: | 6VC9 |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.25 |
| Reference: | 10.1074/jbc.RA120.012395 |
| Antibody | |
| Antibody: | TB19 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | 5'-nucleotidase, ecto (CD73), isoform CRA_a |
| Antigen mutation: | No |
| Durg Target: | P21589 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6VC9_H|Chain A[auth H]|TB19 heavy chain|Homo sapiens (9606) QEQLQESGPGLVKPSETLSLTCTVSGGSIRNNYYNWIRQPAGKGLEWIGRIYISGTTNSNPSLKSRVTMSIDTSKNQFSLKLSSVTAADTAIYYCAREHYVSGTSLDNWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTHHHHHH |
Light Chain: L
Mutation: NULL
| >6VC9_L|Chain B[auth L]|TB19 light chain|Homo sapiens (9606) EIVMTQSPTTLSLSPGERATLSCRASQSVNTNYFSWYQQKPGLTPRLLIYGTSTRATGIPARFSGSGSGTDFTLTISSLQPEDFGIYYCQQDYNLPYTFGQGTYLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >6VC9_A|Chain C[auth A]|5'-nucleotidase, ecto (CD73), isoform CRA_a|Homo sapiens (9606) WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHADEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIKFSHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: ASN31 ASN32 TYR33 ARG50 TYR52 SER54 THR56 ASN58 SER59 PRO61 SER62 LYS64 GLU98 TYR100 VAL101 SER102 GLY103 THR104 L: GLU1 ILE2 VAL3 SER26 GLN27 ASN30 ASN32 TYR33 ASP92 TYR93 ASN94 LEU95 TYR97 A: ASP121 ASN122 GLY123 VAL124 GLU125 LYS145 ALA146 LYS147 GLY148 PRO149 LYS179 GLU180 PRO182 PHE183 SER185 ASN186 PRO187 GLY188 THR189 ASN190 LEU191 VAL192 PHE193 GLU194 ASP195 ARG374 ARG517 ASP519 SER520 GLY521 ASP522 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
