Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2971 |
| PDBID: | 6V4O |
| Chains: | AB_I |
| Organism: | Influenza B virus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 2.80 |
| Reference: | 10.1016/j.immuni.2020.08.015 |
| Antibody | |
| Antibody: | 2E01 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza B virus neuraminidase |
| Antigen mutation: | No |
| Durg Target: | P27907; P03474; |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >6V4O_A|Chain E[auth A], F[auth C], G[auth D], H|Antibody Fab heavy chain|Homo sapiens (9606) EVQLVQSGAEVKKPGSSVKVSCKASGYTFINHALSWVRQAPGQGLEWVGGIIPIFGLAKYGQKFQDRVTITADESTKTAYMDLRSLRSDDTAVYYCARDTVAVYEDFDWSSPYFFYMDVWGKGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCRSLVPRGSSGHHHHHH |
Light Chain: B
Mutation: NULL
| >6V4O_B|Chain I[auth B], J[auth E], K[auth G], L|Antibody Fab light chain|Homo sapiens (9606) EIVLTQSPATLSLFPGERATLSCRASQSAGSKSLAWYQHKVGQPPRLLINGASSRATGIPDRFSGSGSGPDFNLTISRLEPEDFAVYYCQRYGTSLVTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: I
Mutation: NULL
| >6V4O_I|Chain A[auth I], B[auth M], C[auth N], D[auth W]|Neuraminidase|Influenza B virus (11520) ADPHHHHHHSSSDYSDLQRVKQELLEEVKKELQKVKEEIIEAFVQELRKRGSLVPRGSPSRWTYPRLSCPGSTFQKALLISPHRFGETKGNSAPLIIREPFIACGPKECKHFALTHYAAQPGGYYNGTREDRNKLRHLISVKLGKIPTVENSIFHMAAWSGSACHDGREWTYIGVDGPDSNALLKIKYGEAYTDTYHSYAKNILRTQESACNCIGGDCYLMITDGPASGISECRFLKIREGRIIKEIFPTGRVKHTEECTCGFASNKTIECACRDNSYTAKRPFVKLNVETDTAEIRLMCTKTYLDTPRPNDGSITGPCESDGDEGSGGIKGGFVHQRMASKIGRWYSRTMSKTKRMGMGLYVKYDGDPWTDSEALALSGVMVSMEEPGWYSFGFEIKDKKCDVPCIGIEMVHDGGKTTWHSAATAIYCLMGSGQLLWDTVTGVNMTL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: TYR27 THR28 ASN31 HIS32 GLU73 ARG94 THR96 VAL97 ALA98 B: SER30 LYS31 SER32 ASN49 GLY50 SER52 SER53 SER67 TYR91 I: ARG116 HIS134 ALA137 PRO139 ARG147 ARG150 ILE221 ARG223 ALA245 SER246 GLU275 ARG292 ASN294 PRO326 PRO328 ASN329 SER332 THR334 ASP342 GLU343 SER345 GLY346 GLY347 THR372 LYS373 ARG374 TRP408 TYR409 ASP432 HIS439 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
