Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2968 |
| PDBID: | 6V4N |
| Chains: | CG_M |
| Organism: | Influenza B virus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 2.50 |
| Reference: | 10.1016/j.immuni.2020.08.015 |
| Antibody | |
| Antibody: | 1G05 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza B virus neuraminidase |
| Antigen mutation: | No |
| Durg Target: | P27907; P03474; |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >6V4N_C|Chain E[auth A], F[auth C], G[auth D], H|Antibody Fab heavy chain|Homo sapiens (9606) QVQLQESGPGLVRPSETLSLTCTVSGDSIGGSYWNWIRQPPGKGLQWIGYIYYTGITNYNPSLKSRVTMSLDTSKNQISLKMDSVTAADTALYFCARGDYSGYDRDVQVELMDVWGKGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCRSLVPRGSSGHHHHHH |
Light Chain: G
Mutation: NULL
| >6V4N_G|Chain I[auth B], J[auth E], K[auth G], L|Antibody Fab light chain|Homo sapiens (9606) DIQMTQSPSSLSASVRDKVTFVCRASQTISIFLNWYQHKPGEAPKLLIYAASRLQSGVPSRFSGSGSGTDFTLTISGLQPEDFATYYCQQSYSAPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: M
Mutation: NULL
| >6V4N_M|Chain A[auth I], B[auth M], C[auth N], D[auth W]|Neuraminidase|Influenza B virus (11520) ADPHHHHHHSSSDYSDLQRVKQELLEEVKKELQKVKEEIIEAFVQELRKRGSLVPRGSPSRWTYPRLSCPGSTFQKALLISPHRFGETKGNSAPLIIREPFIACGPKECKHFALTHYAAQPGGYYNGTREDRNKLRHLISVKLGKIPTVENSIFHMAAWSGSACHDGREWTYIGVDGPDSNALLKIKYGEAYTDTYHSYAKNILRTQESACNCIGGDCYLMITDGPASGISECRFLKIREGRIIKEIFPTGRVKHTEECTCGFASNKTIECACRDNSYTAKRPFVKLNVETDTAEIRLMCTKTYLDTPRPNDGSITGPCESDGDEGSGGIKGGFVHQRMASKIGRWYSRTMSKTKRMGMGLYVKYDGDPWTDSEALALSGVMVSMEEPGWYSFGFEIKDKKCDVPCIGIEMVHDGGKTTWHSAATAIYCLMGSGQLLWDTVTGVNMTL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: ASP27 SER28 GLY30 GLY31 SER32 TYR50 TYR52 TYR53 THR54 GLY55 ILE56 ASN58 ARG94 TYR97 G: PHE32 TYR49 ARG53 M: ARG116 GLU117 ARG147 GLU148 ASP149 ARG150 TRP177 SER178 ILE221 ARG223 GLU226 ALA245 SER246 GLU276 ARG292 ASN294 SER345 THR372 LYS373 ARG374 MET375 MET403 GLU404 PRO406 TRP408 TYR409 VAL430 ASP432 GLY433 GLY434 LYS435 THR436 THR437 ASN463 MET464 THR465 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
