Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2962 |
| PDBID: | 6UYE |
| Chains: | JL_C |
| Organism: | Ebola virus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.96 |
| Reference: | 10.1016/j.immuni.2020.01.001 |
| Antibody | |
| Antibody: | rEBOV-548 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Ebola virus envelope glycoprotein |
| Antigen mutation: | No |
| Durg Target: | Q05320 |
Sequence information
Antibody
Heavy Chain: J
Mutation: NULL
| >6UYE_J|Chain D[auth J], H[auth K], L|Antibody rEBOV-548 Fab, light chain|Homo sapiens (9606) IQMTQSPSSVSASVGDRVTITCRASQDISNWLAWYQQKPGKAPELLIYTASILQSGVSSRFSGSGSGTDFTLTISSLQPEDSATYYCQQGKSFPYTFGQGTKLEIKRT |
Light Chain: L
Mutation: NULL
| >6UYE_L|Chain D[auth J], H[auth K], L|Antibody rEBOV-548 Fab, light chain|Homo sapiens (9606) IQMTQSPSSVSASVGDRVTITCRASQDISNWLAWYQQKPGKAPELLIYTASILQSGVSSRFSGSGSGTDFTLTISSLQPEDSATYYCQQGKSFPYTFGQGTKLEIKRT |
Antigen
Chain: C
Mutation: NULL
| >6UYE_C|Chain A, E[auth B], I[auth C]|SGP|Ebola virus (1570291) SIPLGVIHNSTLQVSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSVTKRWGFRSGVPPKVVNYEAGEWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFFLYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTIRYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYASGKRSNTTGKLIWKVNPEIDTTIGEWAFWE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ILE2 GLN27 TRP32 TYR49 THR50 SER52 ILE53 LYS92 SER93 C: PRO116 ASP117 GLY118 THR144 LEU239 THR269 THR270 GLY271 LYS272 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
