Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2960 |
| PDBID: | 6UYE |
| Chains: | HK_B |
| Organism: | Ebola virus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.96 |
| Reference: | 10.1016/j.immuni.2020.01.001 |
| Antibody | |
| Antibody: | rEBOV-548 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Ebola virus envelope glycoprotein |
| Antigen mutation: | No |
| Durg Target: | Q05320 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6UYE_H|Chain C[auth G], G[auth H], K[auth I]|Antibody rEBOV-548 Fab, heavy chain|Homo sapiens (9606) QVQVEESGGGVVQPGGSLRLSCAASGFMFSNYGMHWVRQAPGKGLEWMAFIRYDDSKKFYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTALYYCAKELLQVYTSAWGEGHSYYYALDVWGLGTAVTVSSA |
Light Chain: K
Mutation: NULL
| >6UYE_K|Chain D[auth J], H[auth K], L|Antibody rEBOV-548 Fab, light chain|Homo sapiens (9606) IQMTQSPSSVSASVGDRVTITCRASQDISNWLAWYQQKPGKAPELLIYTASILQSGVSSRFSGSGSGTDFTLTISSLQPEDSATYYCQQGKSFPYTFGQGTKLEIKRT |
Antigen
Chain: B
Mutation: NULL
| >6UYE_B|Chain A, E[auth B], I[auth C]|SGP|Ebola virus (1570291) SIPLGVIHNSTLQVSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSVTKRWGFRSGVPPKVVNYEAGEWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFFLYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTIRYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYASGKRSNTTGKLIWKVNPEIDTTIGEWAFWE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ARG52 TYR53 LYS57 GLN102 TYR104 SER106 ALA107 TRP108 GLY109 GLU110 GLY111 HIS112 SER113 TYR114 TYR115 TYR116 K: ILE2 GLN27 TRP32 TYR49 THR50 SER52 ILE53 LYS92 SER93 B: PRO116 ASP117 GLY118 THR144 GLU229 GLU231 LEU239 GLN243 LEU256 THR259 ILE260 LYS265 ARG266 SER267 ASN268 THR269 THR270 GLY271 LYS272 LEU273 ILE274 TRP275 LYS276 VAL277 ASN278 ILE281 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
