Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2950 |
| PDBID: | 6URM |
| Chains: | HL_F |
| Organism: | Influenza A virus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.65 |
| Reference: | 10.1016/j.celrep.2020.108088 |
| Antibody | |
| Antibody: | LPAF-a.01 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H1N1 hemagglutinin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6URM_H|Chain B[auth D], E[auth H]|The heavy chain of antibody LPAF-a.01|Homo sapiens (9606) QVTLRESGPALVKPTQTLTLTCTFSGFSLSTSGMCLTWIRRPPGKALEWLALIDWDDNKYYNTSLRTRLTISKDTSKNQVVLKVTDMDPVDTGTYYCARMLYGDLGGRFDPWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDK |
Light Chain: L
Mutation: NULL
| >6URM_L|Chain C[auth E], F[auth L]|The light chain of antibody LPAF-a.01|Homo sapiens (9606) SYELTQPLSVSVALGQTARITCGGNNIGIKNVHWYQQRPGQAPVLVIYTDINRPSGIPERFSGSNSGNTATLIISKAQAGDEADYFCQVWDSNTVVFGGGTKLTVLSQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHKSYSCQVTHEGSTVEKTVAPTEC |
Antigen
Chain: F
Mutation: NULL
| >6URM_F|Chain A[auth C], D[auth F]|Hemagglutinin|Influenza A virus (11320) DTLCIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDKHNGKLCKLRGVAPLHLGKCNIAGWILGNPECESLSTASSWSYIVETPSSDNGTCYPGDFIDYEELREQLSSVSSFERFEIFPKTSSWPNHDSNKGVTAACPHAGAKSFYKNLIWLVKKGNSYPKLSKSYINDKGKEVLVLWGIHHPSTSADQQSLYQNADTYVFVGSSRYSKKFKPEIAIRPKVRDQEGRMNYYWTLVEPGDKITFEATGNLVVPRYAFAMERNAGSGIIISDTPVHDCNTTCQTPKGAINTSLPFQNIHPITIGKCPKYVKSTKLRLATGLRNIPSIQSRGLFGAIAGFIEGGWTGMVDGWYGYHHQNEQGSGYAADLKSTQNAIDEITNKVNSVIEKMNTQFTAVGKEFNHLEKRIENLNKKVDDGFLDIWTYNAELLVLLENERTLDYHDSNVKNLYEKVRSQLKNNAKEIGNGCFEFYHKCDNTCMESVKNGTYDYPKYSEEAKLNREEIDGVSG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR31 SER32 GLY33 MET34 TRP53 LEU96 TYR97 GLY98 ASP99 L: GLY29 ILE30 LYS31 ASN32 TYR49 THR50 ASP51 ILE52 ASN53 ARG54 SER56 ASN66 SER67 GLY68 ASN94 F: TYR98 ASP131 LYS133 VAL135 ALA137 PRO140 ALA142 GLY143 ALA144 LYS145 VAL155 LYS156 GLY158 ASN159 HIS183 SER186 THR187 SER188 ALA189 ASP190 GLN192 SER193 LEU194 ILE217 ILE219 LYS222 ASP225 GLN226 GLU227 GLY228 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
