Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2944 |
| PDBID: | 6UMX |
| Chains: | hl_B |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.79 |
| Reference: | 10.1074/jbc.RA119.012293 |
| Antibody | |
| Antibody: | GL29H4-16 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Growth/differentiation factor 8 |
| Antigen mutation: | No |
| Durg Target: | O14793 |
Sequence information
Antibody
Heavy Chain: h
Mutation: NULL
| >6UMX_h|Chain C[auth H], E[auth h]|GL29H4-16 Fab Heavy Chain,GL29H4-16 Fab Heavy Chain|Homo sapiens (9606) QIQLVQSGGGVVQPGRSLRLSCAASGFTFSSYGMHWVRQAPGKGLEWVAVISYDGSRKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARDLLVRFLYWSHYYGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: l
Mutation: NULL
| >6UMX_l|Chain B[auth L], D[auth l]|GL29H4-16 Fab Light Chain,GL29H4-16 Fab Light Chain|Homo sapiens (9606) QPVLTQPPSASGTPGQRVTISCSGSSSNIGSNPVHWYQQLPGTAPKLLIYDDNQRPSGVPDRFSGSKSGTSASLVISGLQSDDEADYYCAAWDDSLNSVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: B
Mutation: NULL
| >6UMX_B|Chain A, F[auth B]|Growth/differentiation factor 8|Homo sapiens (9606) HHHHHHENLYFQSNENSEQKENVEKEGLCNACTWRQNTKSSRIEAIKIQILSKLRLETAPNISKDVIRQLLPKAPPLRELIDQYDVQRADSSDGSLEDDDYHATTETIITMPTESDFLMQVDGKPKCCFFKFSSKIQYNKVVKAQLWIYLRPVETPTTVFVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIWQSIDVKTVLQNWLKQPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVTDTPKASRADFGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEFVFLQKYPHTHLVHQANPRGSAGPCCTPTKMSPINMLYFNGKEQIIYGKIPAMVVDRCGCS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| l: TYR50 ASP51 ASP52 ASN53 GLN54 PRO56 SER57 h: PHE27 THR28 SER31 TYR32 ARG98 LEU100 LEU101 VAL102 PHE104 LEU105 TYR106 TRP107 TYR111 ASP114 B: PHE170 VAL171 GLN172 LEU174 ARG185 TYR186 THR187 GLY188 ILE189 ARG190 SER191 LEU192 LYS193 ASP195 GLN204 SER205 ASP207 LYS228 LEU230 ASP231 GLU232 ASN233 GLY234 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
