Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2942 |
| PDBID: | 6UM7 |
| Chains: | BC_I |
| Organism: | Human immunodeficiency virus 1, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.50 |
| Reference: | 10.1126/science.aay7199 |
| Antibody | |
| Antibody: | DH270.mu1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 CH848 10.17 DT gp120 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >6UM7_B|Chain C[auth B], G[auth F], J|DH270.mu1 Fab Heavy Chain|Homo sapiens (9606) MGWSCIILFLVATATGVHSQVQLVQSGAEVKKPGASVKVSCKASGYTFTDYYIHWVRQAPGQGLEWMGWINPNSGRTNSAQKFQDRVTMTRDTSITTASMELSRLTSDDTAVYYCARGGWISLYYDSSGYPNFNYWGQGSRVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDK |
Light Chain: C
Mutation: NULL
| >6UM7_C|Chain D[auth C], H[auth G], K|DH270.mu1 Fab Light chain|Homo sapiens (9606) MGWSCIILFLVATATGSWAQSALTQPASVSGSPGQSITISCTGTSYDVGTYNLVSWYQQHPGKAPKLMIYEVSKRPSGVSFRFSGSKSGNTASLTISGLQAEDAADYYCCSYAGSATVIFGGGSKMTVLGQPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: I
Mutation: NULL
| >6UM7_I|Chain A, F[auth E], L[auth I]|Envelope glycoprotein gp120|Human immunodeficiency virus 1 (11676) AENLWVTVYYGVPVWKEAKTTLFCASDARAYEKEVHNVWATHACVPTDPSPQELVLGNVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLICSDATVKTGTVEEMKNCSFNTTTEIRDKEKKEYALFYKPDIVPLSETNNTSEYRLINCNTSACTQACPKVTFEPIPIHYCAPAGYAILKCNDETFNGTGPCSNVSTVQCTHGIRPVVSTQLLLNGSLAEKEIVIRSENLTNNAKIIIVHLHTPVEIVCTRPNNNTRKSVRIGPGQTFYATGDIIGDIKQAHCNISEEKWNDTLQKVGIELQKHFPNKTIKYNQSAGGDMEITTHSFNCGGEFFYCNTSNLFNGTYNGTYISTNSSANSTSTITLQCRIKQIINMWQGVGRCMYAPPIAGNITCRSNITGLLLTRDGGTNSNETETFRPAGGDMRDNWRSELYKYKVVKIEPLGVAPTRCKRRV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: TYR33 TRP50 ASN52 ASN54 SER55 GLY56 ARG57 ASN59 ARG72 ILE102 SER103 LEU104 TYR105 TYR106 ASP107 SER109 C: SER96 ALA97 I: THR135 LYS137 THR138 THR148 VAL149 PRO299 ASN300 ASN301 ASP321 ILE322 ILE323 GLY324 ASP325 ILE326 LYS327 GLN328 HIS330 THR415 GLN417 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
