Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2931 |
| PDBID: | 6UKT |
| Chains: | F_B |
| Organism: | Rattus norvegicus, Lama glama |
| Method: | EM |
| Resolution (Å): | 3.87 |
| Reference: | 10.1038/s41467-020-14943-4 |
| Antibody | |
| Antibody: | NB9156 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Guanine nucleotide-binding protein G(i) subunit alpha-1 |
| Antigen mutation: | No |
| Durg Target: | P63096 |
Sequence information
Antibody
Chain: F
Mutation: NULL
| >6UKT_F|Chain F|NB9156|Lama glama (9844) QVQLQESGGGLVQAGGSLRLSCAASVRTSDTDGMAWFRQAPGKEREFVGGIRWNSATWYADFVKGRFTISRDNAKNTLYLQMNSLKPEDTALYYCARRAYGFDTDSRESAYSNWGQGTQVTVSSHHHHHHEPEA |
Antigen
Chain: B
Mutation: NULL
| >6UKT_B|Chain B|Guanine nucleotide-binding protein G(i) subunit alpha-1|Rattus norvegicus (10116) REVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDAARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: ARG27 ASP30 TRP53 ASN54 SER55 ALA56 ALA74 TYR100 GLY101 PHE102 B: ARG86 MET88 GLY89 ARG90 LYS92 ILE93 ASP94 ALA101 GLU239 LEU283 THR284 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
