Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2930 |
| PDBID: | 6UKT |
| Chains: | E_A |
| Organism: | Rattus norvegicus, Lama glama |
| Method: | EM |
| Resolution (Å): | 3.87 |
| Reference: | 10.1038/s41467-020-14943-4 |
| Antibody | |
| Antibody: | NB8119 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Chain: E
Mutation: NULL
| >6UKT_E|Chain E|NB8119|Lama glama (9844) QVQLQESGGGLVQAGGSLRLSCAASGGIVHISSMGWFRQAPGKQRELVATSPSNGDIRYADSVKGRFTLSRDNAKNTVSLQMNSLEPEDTAVYYCHSFLRHTASASYNNYYGQGTQVTVSSHHHHHHEPEA |
Antigen
Chain: A
Mutation: Y233F
| >6UKT_A|Chain A|Resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)|Rattus norvegicus (10116) GMEPRAVADALETGEEDAVTEALRSFNREHSQSFTFDDAQQEDRKRLAKLLVSVLEQGLSPKHRVTWLQTIRILSRDRSCLDSFASRQSLHALACYADIAISEEPIPQPPDMDVLLESLKCLCNLVLSSPTAQMLAAEARLVVRLAERVGLYRKRSYPHEVQFFDLRLLFLLTALRTDVRQQLFQELHGVRLLTDALELTLGVAPKENPLVILPAQETERAMEILKVLFNITFDSVKREVDEEDAALYRYLGTLLRHCVMADAAGDRTEEFHGHTVNLLGNLPLKCLDVLLALELHEGSLEFMGVNMDVINALLAFLEKRLHQTHRLKECVAPVLSVLTECARMHRPARKFLKAQVLPPLRDVRTRPEVGDLLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYGNAAGLLAARGLMAGGRPEGQYSEDEDTDTEEYREAKASINPVTGRVEEKPPNPMEGMTEEQKEHEAMKLVNMFDKLSR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: SER33 GLY35 TRP36 PHE37 GLN44 GLU46 LEU47 THR50 PRO52 ASN54 GLY55 ASP56 ARG58 ASP61 HIS96 SER97 PHE98 SER106 TYR107 ASN108 TYR111 A: GLU2 PRO3 ARG4 PHE33 THR34 PHE35 ASP36 ASP37 ALA38 GLN40 GLU41 ASP42 ASP76 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
