Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 293 |
| PDBID: | 2KH2 |
| Chains: | B_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | NMR |
| Resolution (Å): | |
| Reference: | 10.1074/jbc.M109.025304 |
| Antibody | |
| Antibody: | 2KH2 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-1 beta |
| Antigen mutation: | No |
| Durg Target: | P01584 |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >2KH2_B|Chain B|scFv|Mus musculus (10090) DIQMTQSPSSLSASVGDRVTITCRTSGNIHNYLTWYQQKPGKAPQLLIYNAKTLADGVPSRFSGSGSGTQFTLTISSLQPEDFANYYCQHFWSLPFTFGQGTKVEIKRTGGGGSGGGGSGGGGSGGGGSEVQLVESGGGLVQPGGSLRLSCAASGFDFSRYDMSWVRQAPGKRLEWVAYISSGGGSTYFPDTVKGRFTISRDNAKNTLYLQMNSLRAEDTAVYYCARQNKKLTWFDYWGQGTLVTVSSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >2KH2_A|Chain A|Interleukin-1 beta|Homo sapiens (9606) APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: ASP1 ASN28 ILE29 HIS30 ASN31 TYR32 TRP92 SER93 LEU94 PHE96 ASP162 TYR179 SER181 SER182 GLY183 GLY184 GLY185 SER186 THR187 TYR188 PHE189 PRO190 ASP191 THR192 LYS194 LYS231 A: ALA1 ARG4 SER5 LEU6 ARG11 GLN15 MET36 GLU37 GLN39 PHE46 ASN53 ASP54 GLU105 ASN108 LYS109 LEU110 THR147 MET148 GLN149 PHE150 VAL151 SER152 SER153 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
