Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2928 |
| PDBID: | 6UKT |
| Chains: | C_A |
| Organism: | Rattus norvegicus, Lama glama |
| Method: | EM |
| Resolution (Å): | 3.87 |
| Reference: | 10.1038/s41467-020-14943-4 |
| Antibody | |
| Antibody: | NB8109 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Chain: C
Mutation: NULL
| >6UKT_C|Chain C|NB8109|Lama glama (9844) QVQLQESGGGLVQPGGSLRLSCAASGIIFRSNGMAWYRQAPGKEREWVASITSFGDAIYRDSVKGRFTISRDNARNAVSLQTNSLKTEDTAVYYCNTYPVNSAWGQGTQVTVSSHHHHHHEPEA |
Antigen
Chain: A
Mutation: Y233F
| >6UKT_A|Chain A|Resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)|Rattus norvegicus (10116) GMEPRAVADALETGEEDAVTEALRSFNREHSQSFTFDDAQQEDRKRLAKLLVSVLEQGLSPKHRVTWLQTIRILSRDRSCLDSFASRQSLHALACYADIAISEEPIPQPPDMDVLLESLKCLCNLVLSSPTAQMLAAEARLVVRLAERVGLYRKRSYPHEVQFFDLRLLFLLTALRTDVRQQLFQELHGVRLLTDALELTLGVAPKENPLVILPAQETERAMEILKVLFNITFDSVKREVDEEDAALYRYLGTLLRHCVMADAAGDRTEEFHGHTVNLLGNLPLKCLDVLLALELHEGSLEFMGVNMDVINALLAFLEKRLHQTHRLKECVAPVLSVLTECARMHRPARKFLKAQVLPPLRDVRTRPEVGDLLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYGNAAGLLAARGLMAGGRPEGQYSEDEDTDTEEYREAKASINPVTGRVEEKPPNPMEGMTEEQKEHEAMKLVNMFDKLSR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: ILE27 SER31 ASN32 GLY33 GLU46 TRP47 SER50 ILE51 THR52 SER53 PHE54 GLY55 ASP56 ILE58 TYR59 ARG60 TYR98 PRO99 VAL100 ASN101 A: ARG152 LYS153 ARG190 THR193 ASP194 GLU197 LEU198 LEU200 GLY201 GLU206 ASN207 GLU241 GLU242 ALA245 LEU246 ARG248 TYR249 THR252 LEU253 ARG255 HIS256 VAL288 GLU293 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
