Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2927 |
| PDBID: | 6UKJ |
| Chains: | HL_A |
| Organism: | Plasmodium falciparum 7G8, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.30 |
| Reference: | 10.1038/s41586-019-1795-x |
| Antibody | |
| Antibody: | CTC Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Plasmodium falciparum Chloroquine Resistance Transporter (PfCRT) 7G8 Isoform |
| Antigen mutation: | No |
| Durg Target: | Q9N623 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6UKJ_H|Chain B[auth H]|Fab Heavy Chain|Homo sapiens (9606) EISEVQLVESGGGLVQPGGSLRLSCAASGFNVSYSYIHWVRQAPGKGLEWVASIYPYSGYTSYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARYGSNYSFWYRGSSVTYAIDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTC |
Light Chain: L
Mutation: NULL
| >6UKJ_L|Chain C[auth L]|Fab Light Chain|Homo sapiens (9606) SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQSSTWPITFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >6UKJ_A|Chain A|Chloroquine resistance transporter|Plasmodium falciparum (isolate 7G8) (57266) MGRAKFASKKNNQKNSSKNDERYRELDNLVQEGNGSRLGGGSCLGKCAHVFKLIFKEIKDNIFIYILSIIYLSVSVMNTIFAKRTLNKIGNYSFVTSETHNFICMIMFFIVYSLFGNKKGNSKERHRSFNLQFFAISMLDACSVILAFIGLTRTTGNIQSFVLQLSIPINMFFCFLILRYRYHLYNYLGAVIIVVTIALVEMKLSFETQEENSIIFNLVLISSLIPVCFSNMTREIVFKKYKIDILRLNAMVSFFQLFTSCLILPVYTLPFLKQLHLPYNEIWTNIKNGFACLFLGRNTVVENCGLGMAKLCDDCDGAWKTFALFSFFDICDNLITSYIIDKFSTMTYTIVSCIQGPALAIAYYFKFLAGDVVREPRLLDFVTLFGYLFGSIIYRVGNIILERKKMRNEENEDSEGELTNVDSIITQAAAGGGSGGGSENLYFQGSHHHHHHHHHHWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TYR34 SER35 TYR36 TYR55 TYR57 SER58 TYR60 ARG101 TYR102 GLY103 SER104 ASN105 TYR106 SER107 PHE108 TRP109 TYR110 ARG111 GLY112 SER113 SER114 VAL115 THR116 TYR117 ALA118 ASP120 TYR121 L: SER29 SER31 SER32 LEU47 TYR50 SER53 TYR56 SER57 ARG67 GLY69 THR70 SER92 SER93 THR94 TRP95 ILE97 A: SER72 ASN75 THR76 ALA79 LYS80 LEU83 ASN84 SER90 THR93 SER94 VAL141 ALA144 PHE145 LEU148 THR149 ARG150 THR151 GLY153 GLN156 PHE203 GLU204 THR205 GLN206 GLU207 GLU208 ASN209 SER210 ILE212 PHE213 PRO267 PHE268 LEU269 LYS270 GLN271 LEU272 ASN300 CYS301 GLY302 LEU303 GLY304 MET305 LEU308 PHE322 ASP329 GLN352 LEU356 TYR360 ASP368 VAL369 VAL370 ARG371 GLU372 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
