Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2895 |
| PDBID: | 6U54 |
| Chains: | A_B |
| Organism: | Lama glama, Ebola virus - Zaire (1995) |
| Method: | XRD |
| Resolution (Å): | 1.60 |
| Reference: | 10.1016/j.jmb.2019.10.005 |
| Antibody | |
| Antibody: | Zaire C (ZC) VH-sdAb |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Zaire ebolavirus Nucleoprotein C-terminal Domain |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: A
Mutation: NULL
| >6U54_A|Chain A|Anti-Zaire ebolavirus Nucleoprotein Single Domain Antibody Zaire C (ZC)|Lama glama (9844) KVQLQQSGGGSVTPGGSLRLSCAASGSISDFAAMAWYRQAPGKERDWVGTIFSAGALLYAEPVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCRLYAEAIYWGQGTQVTVSS |
Antigen
Chain: B
Mutation: NULL
| >6U54_B|Chain B|Nucleoprotein|Zaire ebolavirus (strain Kikwit-95) (128951) KIHHHHHHGGGSARNQDSDNTQPEHSFEEMYRHILRSQGPFDAVLYYHMMKDEPVVFSTSDGKEYTYPDSLEEEYPPWLTEKEAMNEENRFVTLDGQQFYWPVMNHKNKFMAILQHHQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: SER29 ASP30 ALA32 ALA33 ALA35 TYR37 ARG45 TRP47 THR50 PHE52 SER53 ALA54 ALA56 LEU58 TYR59 GLU61 LYS64 ARG96 TYR98 ALA99 GLU100 ALA101 ILE102 B: GLU645 HIS646 MET651 HIS654 ILE655 GLN659 PHE662 ASP663 LEU666 TYR667 HIS669 MET670 MET671 ASP673 TYR688 PRO689 GLU693 VAL724 MET725 ASN726 HIS727 LYS728 LYS730 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
