Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2877 |
| PDBID: | 6TYS |
| Chains: | HL_A |
| Organism: | Henipavirus nipahense, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.50 |
| Reference: | 10.1038/s41594-019-0308-9 |
| Antibody | |
| Antibody: | 5B3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Henipavirus nipahense Fusion glycoprotein F0 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6TYS_H|Chain B[auth C], E[auth F], H|5B3 antibody heavy chain|Mus musculus (10090) EVQLVESGGGLVKPGGSLKLSCAASGFTFSSYDMSWVRQTPEKRLEWVAMISSGGSYSYYPDSVKGRFTISRDNAKNTLYLQMSSLRSEDTAMYYCARQGDYAWFAYWGQGTLVTVSSAS |
Light Chain: L
Mutation: NULL
| >6TYS_L|Chain C[auth D], F[auth G], I[auth L]|5B3 antibody light chain|Mus musculus (10090) DIQMTQSPASQSASLGESVTITCLASQTIGTWLAWYQQKPGKSPQLLIYAATSLADGVPSRFSGSGSGTKFSFKISSLQAEDFVSYYCQQFYSTPFTFGGGTKLEIK |
Antigen
Chain: A
Mutation: NULL
| >6TYS_A|Chain A, D[auth B], G[auth E]|Fusion glycoprotein F0|Nipah virus (3052225) MVVILDKRCYCNLLILILMISECSVGILHYEKLSKIGLVKGVTRKYKIKSNPLTKDIVIKMIPNVSNMSQCTGSVMENYKTRLNGILTPIKGALEIYKNCTHDLDYKDDDDKVGDVRLAGVIMAGVCIGIATAAQITAGVALYEAMKNADNINKLKSSIESTNEAVVKLQETAEKTVYVLTALQDYINTNLVPTIDKISCKQTELSLDLALSKYLSDLLFVFGPNLQDPVSNSMTIQAISQAFGGNYETLLRTLGYATEDFDDLLESDSITGQIIYVDLSSYYIIVRVYFPILTEIQQAYIQELLPVSFNNDNSEWISIVPNFILVRNTLISNIEIGFCLITKRSVICNQDYATPMTNNMRECLTGSTEKCPRELVVSSHVPRFALSNGVLFANCISVTCQCQTTGRAISQSGEQTLLMIDNTTCPTAVLGNVIISLGKYLGSVNYNSEGIAIGPPVFTDKVDISSQISSMNQSLQQSKDYIKEAQRLLDTVNPSLISMLSMMKQIEDKIEEILSKIYHIENEIARIKKLIGERIDGR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR28 SER30 SER31 ASP33 MET50 SER52 SER53 GLY54 SER56 TYR57 TYR59 GLN99 ASP101 TYR102 ALA103 TRP104 L: ALA25 SER26 GLN27 THR28 GLY30 THR31 TRP32 TYR49 ALA50 SER53 SER67 GLY68 THR69 PHE91 TYR92 SER93 THR94 PHE96 A: LYS45 ASN51 LEU53 THR54 LYS55 ASP56 LYS139 ASN140 LYS160 GLN162 ALA165 GLU166 THR168 LEU246 GLY247 TYR248 ALA249 THR250 GLU251 ASP252 PHE253 ASP254 LEU256 PHE282 PRO283 ILE284 LEU285 ARG336 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
