Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2876 |
| PDBID: | 6TYL |
| Chains: | D_A |
| Organism: | Rattus norvegicus, Lama glama |
| Method: | XRD |
| Resolution (Å): | 3.30 |
| Reference: | 10.1038/s41467-020-14943-4 |
| Antibody | |
| Antibody: | NB8117 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >6TYL_D|Chain F[auth D], G[auth I]|Nanobody C|Lama glama (9844) QVQLQESGGGLEQAGDSLRLSCAASGLIVSNYAMGWFRQAPGKEREFVAYINWNGGVTYYTNSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCARTSRASVTTRVADFGYWGQGTQVTVSSHHHHHHEPEA |
Antigen
Chain: A
Mutation: NULL
| >6TYL_A|Chain A, J[auth F]|Resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)|Rattus norvegicus (10116) GMEPRAVADALETGEEDAVTEALRSFNREHSQSFTFDDAQQEDRKRLAKLLVSVLEQGLSPKHRVTWLQTIRILSRDRSCLDSFASRQSLHALACYADIAISEEPIPQPPDMDVLLESLKCLCNLVLSSPTAQMLAAEARLVVRLAERVGLYRKRSYPHEVQFFDLRLLFLLTALRTDVRQQLFQELHGVRLLTDALELTLGVAPKENPLVILPAQETERAMEILKVLFNITFDSVKREVDEEDAALYRYLGTLLRHCVMADAAGDRTEEFHGHTVNLLGNLPLKCLDVLLALELHEGSLEFMGVNMDVINALLAFLEKRLHQTHRLKECVAPVLSVLTECARMHRPARKFLKAQVLPPLRDVRTRPEVGDLLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYGNAAGLLAARGLMAGGRPEGQYSEDEDTDTEEYREAKASINPVTGRVEEKPPNPMEGMTEEQKEHEAMKLVNMFDKLSR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: VAL29 ASN31 TYR32 ASN52 TRP53 ASN54 GLY56 VAL57 THR58 TYR59 ASN62 LYS65 ASN74 ARG98 SER100 ARG101 ALA102 SER103 VAL104 THR105 A: GLU296 GLY297 SER298 LEU299 GLU300 PHE301 ASP307 ASN310 LEU313 ALA314 GLU317 LYS318 PHE350 ALA353 GLN354 VAL355 PRO357 PRO358 LEU359 ARG360 ASP361 VAL362 ASP370 LEU371 LEU372 GLU438 THR442 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
